Reference : Somatic embryogenesis of Panax ginseng in liquid cultures: a role for polyamines and ...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
http://hdl.handle.net/2268/3296
Somatic embryogenesis of Panax ginseng in liquid cultures: a role for polyamines and their metabolic pathways
English
Kevers, Claire mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Le Gal, Nathalie [Université de Liège - ULiège > > Hormonologie végétale > >]
Monteiro, Marta [Université de Liège - ULiège > > Hormonologie végétale > >]
Dommes, Jacques mailto [Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales >]
Gaspar, Thomas mailto [> > Botanique, radiobotanique & serres expér. >]
2000
Plant Growth Regulation
Kluwer Academic Publishers
31
3
209-214
Yes
International
0167-6903
1573-5087
Dordrecht
The Netherlands
[en] polyamines ; somatic embryogenesis ; Panax ginseng
[en] A callus with embryogenic capacity was generated from root sections of Panax ginseng and used as an inoculum source for embryogenic liquid cultures in a three-step process: - a suspension culture of cell aggregates in the presence of an auxin/cytokinin mixture, - an induction medium containing auxin only (for 5 to 30 days), - a regeneration medium containing cytokinin only (for one month). Up to 25 embryos were recovered per 2.5 g of aggregates in these conditions. Incorporation of polyamines or their precursors arginine and ornithine into either the induction or regeneration media increased the number of embryos produced by up to 4 times. Inhibitors of both biosynthesis and biodegradation of polyamines reduced the number of embryos. These results support earlier findings of the role of polyamines in the process of somatic embryogenesis. The success of these liquid cultures opens up the possibility of producing somatic embryos of Panax ginseng in bioreactors.
Researchers
http://hdl.handle.net/2268/3296
10.1023/A:1006344316683

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