Hydrogen peroxide; Nf-kb; lymphocytes; Signal transduction; free radical
Résumé :
[en] A large body of work has been devoted to mechanisms leading to the activation of the transcription factor NF-kappa B in various cell types. Several studies have indicated that NF-kappa B activation by numerous stimuli depends on the intracellular generation of reactive oxygen species (ROS). In this report, we first demonstrated that inhibition of the electron transport chain by either rotenone or antimycine A gave rise to dose-dependent inhibition of NF-kappa B translocation induced by 150 microM of hydrogen peroxide (H2O2). Conversely, the impairment of the mitochondrial respiratory chain did not affect T lymphocyte treatment by TNF-alpha (tumor necrosis factor alpha) or pre-B lymphocyte treatment with LPS (lipopolysaccharide). We also showed that oligomycine which inhibits ATP synthase and FCCP, which uncouples respiration also led to dose-dependent inhibition of NF-kappa B activation by H2O2. All these inhibitors were also shown to inhibit mitochondrial respiration in lymphocytes assessed by oxygen consumption. Although only a transient drop in ATP concentration was observed when lymphocytes were treated by H2O2, this effect was remarkably reinforced in the presence of oligomycine demonstrating the crucial role of ATP in the signal transduction pathway induced by H2O2.
Disciplines :
Biochimie, biophysique & biologie moléculaire
Auteur, co-auteur :
JOSSE, Claire ; Centre Hospitalier Universitaire de Liège - CHU > Service d'oncologie médicale
Baeuerle P. A., Henkel T. Function and activation of NF-κB in the immune system. Annu. Rev. Immunol. 12:1994;141-179.
Siebenlist U., Franzoso G., Brown K. Structure, regulation, and function of NF-κB. Annu. Rev. Biol. 10:1994;405-455.
Miyamoto S., Verma I. M. Rel/NF-κB/IκB story. Adv. Cancer Res. 66:1995;255-292.
Beg A. A., Baldwin A. S. The IκB proteins multifunctional regulators of Rel/NF-κB transcription factors . Genes Dev. 7:1993;2064-2070.
Haskill S., Beg A. A., Tompkins S. M., Morris J. S., Yurochko A. D., Sampson-Johannes A., Mondal K., Ralph P., Baldwin A. S. J. Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity. Cell. 65:1991;1281-1289.
Mercurio F., DiDonato J. A., Rosette C., Karin M. p105 and p98 precursor proteins play an active role in NF-κB-mediated signal transduction. Genes Dev. 7:1993;705-718.
Rice N. R., MacKichan M. L., Israël A. The precursor of NF-κB p50 has IκB-like functions. Cell. 71:1992;243-253.
Thompson J. E., Phillips R. J., Erdjument-Bromage H., Tempst P., Ghosh S. IκB-β regulates the persistent response in a biphasic activation of NF-κB. Cell. 80:1995;573-582.
Beg A. A., Finco T. S., Nantermet P. V., Baldwin A. S. J. Tumor necrosis factor and interleukine-1 lead to phosphorylation and loss of IκBα a mechanism for NF-κB activation . Mol. Cell. Biol. 13:1993;3301-3310.
Brown K., Park S., Kanno T., Franzoso G., Sibenlist U. Mutual regulation of the transcriptional activator NF-κB and its inhibitor, IκB-α Proc. Natl. Acad. Sci. USA. 90:1993;2532-2536.
Brown K., Gerstberger S., Carlson L., Franzoso G., Siebenlist U. Control of IκB-α proteolysis by site-specific, signal-induced phosphorylation. Science. 267:1995;1485-1488.
Traenckner E. B.-M., Pahl H. L., Henkel T., Schmidt K. N., Wilk S., Baeuerle P. A. Phosphorylation of IκB-α on serines 32 and 36 controls IκB-α proteolysis and NF-κB activation in response to diverse stimuli. EMBO J. 14:1995;2876-2883.
Traenckner E. B.-M., Wilk S., Baeuerle P. A. A proteasome inhibitor prevents activation of NF-κB and stabilizes a newly phosphorylated form of IκB-α that is still bound to NF-κB. EMBO J. 13:1994;5433-5441.
Régnier C. H., Song H. Y., Gao X., Goeddel D. V., Cao Z., Rothe M. Identification and characterization of an IκB kinase. Cell. 90:1997;373-383.
Song H. Y., Régnier C., Kirschning C. J., Goeddel D. V., Rothe M. Tumor necrosis factor (TNF)-mediated kinase cascades bifurcation of nuclear factor-κB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2 . Proc. Natl. Acad. Sci. USA. 94:1997;9792-9796.
DiDonato J. A., Hayakawa D. M., Rothwarf D. M., Zandi E., Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature. 388:1997;548-554.
Mercurio F., Zhu H., Murray B. W., Shevchenko A., Bennett B. L., Li J. W., Young D. B., Barbosa M., Mann B. IKK-2 and IKK-2 Cytokine-activated IκB kinases essential for NF-κB activation . Science. 278:1997;860-866.
Woronicz J. D., Gao X., Cao Z., Rothe M., Goeddel G. V. IκB kinase-β NF-κB activation and complex formation with IκB kinase-α and NIK . Science. 278:1997;866-869.
Satriano J. A., Shuldiner M., Hora K., Xing Y., Shan Z., Schlondorff D. Oxygen radicals as second messengers for expression of the monocyte chemoattractant protein, JE/MCP-1, and the colony-stimulating factor, CSF-1, in response to tumor necrosis factor-alpha and immunoglobulin G. Evidence for involvement of reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent oxidase. J. Clin. Invest. 92:1995;1564-1571.
Feng L., Xia Y., Garcia G. E., Hwang D., Wilson C. B. Involvement of reactive oxygen intermediates in cyclo-oxygenase-2 expression induced by interleukin-1, tumor necrosis factor-alpha, and lipopolysaccharide. J. Clin. Invest. 95:1995;1669-1675.
Legrand-Poels S., Maniglia S., Boelaert J. R., Piette J. Activation of the transcription factor NF-κB in lipopolysaccharide-stimulated U937 cells. Biochem. Pharmacol. 53:1997;339-346.
Legrand-Poels S., Bours V., Piret B., Pflaum M., Epe B., Rentier B., Piette J. Transcription factor NF-κB is activated by photosensitization generating oxidative DNA damages. J. Biol. Chem. 270:1995;6925-6934.
Turrens J. F., Boveris A. Generation of superoxide anion by the NADH deshydrogenase of bovine heart mitochondria. Biochem. J. 191:1980;421-427.
Garcia-Ruiz C., Collel A., Morales A., Kaplowitz N., Fernandez-Checa J. C. Role of oxidative stress generated from the mitochondrial electron transport chain and mitochondrial glutathione status in loss of mitochondrial function and activation of transcription factor nuclear factor-κB studies with isolated mitochondria and rat hepatocytes . Mol. Pharmacol. 48:1995;825-834.
Schreck R., Rieber P., Baeuerle P. A. Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF-kappa B transcription factor and HIV-1. EMBO J. 10:1991;2247-2258.
Schoonbroodt S., Legrand-Poels S., Best-Belpomme M., Piette J. Activation of the NF-κB transcription factor in a T-lymphocytic cell line by hypochlorous acid. Biochem. J. 321:1997;777-785.
Legrand-Poels S., Zecchinon L., Piret B., Schoonbroodt S., Piette J. Involvement of different transduction pathways in NF-κB activation by several inducers. Free Radic. Res. 27:1997;301-309.
Brodie A. E., Reed D. J. Reversible oxidation of glyceraldehyde 3-phosphate dehydrogenase thiols in human lung carcinoma cells by hydrogen peroxide. Biochem. Biophys. Acta. 148:1987;120-125.
Kluck R. M., Bossy-Wetzel E., Green D. R., Newmeyer D. D. The release of cytochrome c from mitochondria a primary site for Bcl-2 regulation of apoptosis . Science. 275:1997;1132-1136.
Yan L.-J., Levine R., Sohal R. S. Oxidative damage during aging targets mitochondrial aconitase. Proc. Natl. Acad. Sci. USA. 94:1997;11168-11172.
Scharaufstatter, I. U.; Hyslop, P. A.; Hinshaw, D. B.; Spragg, R. G.; Sklar, L. A.; Cochrane, C. G. Hydrogen peroxide-induced injury of cells and its prevention by inhibitors of poly (ADP-ribose) polymerase. Proc. Natl. Acad. Sci. USA 83:4908-4912.
Stadtman E. R. Biochemical markers of aging. Exp. Gerontol. 23:1988;327-347.
Schulze-Osthoff K., Beyaert R., Vandevoorde V., Haegeman G., Fiers W. Depletion of the mitochondrial electron transport abrogates the cytotoxic and gene-inductive effects of TNF. EMBO J. 12:1993;3095-3104.
Bonizzi G., Dejardin E., Piret J., Piette J., Merville M.-P., Bours V. IL-1β induces NF-κB in epithelial cells independently of the production of reactive oxygen intermediates. Eur. J. Biochem. 242:1996;544-551.
Bonizzi G., Piette J., Merville M.-P., Bours V. Distinct signal transduction pathways mediate nuclear factor-κB induction in IL-1β in epithelial and lymphoid cells. J. Immunol. 159:1997;5264-5272.
Brennan P., O'Neill L. A. J. Effects of oxidants and antioxidants on nuclear factor κB activation in three different cell lines evidence against a universal hypothesis involving oxygen radicals . Biochim. Biophys. Acta. 1260:1995;167-175.
Bowie A., Moynagh P. N., O'Neill L. A. J. Lipid peroxidation is involved in the activation of NF-κB by tumor necrosis factor but not interleukin-1 in the human endothelial cell line ECV304. J. Biol. Chem. 272:1997;25941-25950.
