Advances in automated and reactive flow cytometry for synthetic biotechnology.

Flow Cytometry; Gene Regulatory Networks; Systems Biology; Synthetic Biology; Biotechnology; Software; Automated flow; Automated flow cytometry; Bioprocess control; Bioprocess monitoring; Bioprocess optimization; Cultivation conditions; Physical interface; Population data; Reactive flow; Software interfaces; Bioengineering; Biomedical Engineering

Abstract :

[en] Automated flow cytometry (FC) has been initially considered for bioprocess monitoring and optimization. More recently, new physical and software interfaces have been made available, facilitating the access to this technology for labs and industries. It also comes with new capabilities, such as being able to act on the cultivation conditions based on population data. This approach, known as reactive FC, extended the range of applications of automated FC to bioprocess control and the stabilization of cocultures, but also to the broad field of synthetic and systems biology for the characterization of gene circuits. However, several issues must be addressed before automated and reactive FC can be considered standard and modular technologies.

Disciplines :

Biotechnology

Author, co-author :

Delvigne, Frank ; Université de Liège - ULiège > TERRA Research Centre > Microbial technologies

Martinez Alvarez, Juan Andrés ; Université de Liège - ULiège > Département GxABT > Microbial technologies

Language :

English

Title :

Advances in automated and reactive flow cytometry for synthetic biotechnology.

Publication date :

October 2023

Journal title :

Current Opinion in Biotechnology

ISSN :

0958-1669

eISSN :

1879-0429

Publisher :

Elsevier Ltd, England

Volume :

Pages :

102974

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://api.elsevier.com/content/article/PII:S0958166923000848?httpAccept=text/xml

Funding text :

This project was funded by SPW (Service Public de Wallonie) and the EU H2020 (European Union´s Horizon 2020) research and innovation programme under grant agreement No [ 722361 ] through a co-found grant acquired during the third call of the Era Cobiotech initiative (Contibio project). The authors are also very grateful to Andrew Zicler for the amazing drawings included in Figure 2 .

Available on ORBi :

since 27 May 2024

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