Article (Scientific journals)
Serum proteome signatures associated with ileal and colonic ulcers in Crohn's disease.
Pierre, Nicolas; Huynh-Thu, Vân Anh; Baiwir, Dominique et al.
2024In Journal of Proteomics, p. 105199
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Keywords :
Colonic ulcers; Crohn's disease; Disease location; Ileal ulcers; Mass spectrometry; Serum proteomics
Abstract :
[en] At a clinical level, ileal and colonic Crohn's disease (CD) are considered as separate entities. These subphenotypes need to be better supported by biological data to develop personalised medicine in CD. To this end, we combined different technologies (proximity extension assay, selected reaction monitoring, and high-sensitivity turbidimetric immunoassay (hsCRP)) to measure 207 immune-related serum proteins in CD patients presenting no endoscopic lesions (endoscopic remission) (n = 23), isolated ileal ulcers (n = 17), or isolated colonic ulcers (n = 16). We showed that isolated ileal ulcers and isolated colonic ulcers were specifically associated with 6 and 18 serum proteins, respectively: (high level: JUN, CNTNAP2; low level: FCRL6, LTA, CLEC4A, NTF4); (high level: hsCRP, IL6, APCS, CFB, MBL2, IL7, IL17A, CCL19, CXCL10, CSF3, IL10, CLEC4G, MMP12, VEGFA; low level: CLEC3B, GSN, TNFSF12, TPSAB1). Isolated ileal ulcers and isolated colonic ulcers was detected by hsCRP with an area under the receiver operating characteristics curve of 0.64 (p-value = 0.07) and 0.77 (p-value = 0.001), respectively. We highlighted distinct serum proteome profiles associated with ileal and colonic ulcers in CD, this finding might support the development of therapeutics and biomarkers tailored to disease location. SIGNIFICANCE: Although ileal and colonic Crohn's disease present important clinical differences (eg, progression, response to treatment and reliability of biomarkers), these two entities are managed with the same therapeutic strategy. The biological specificities of ileal and colonic Crohn's disease need to be better characterised to develop more personalised approaches. The present study used robust technologies (selected reaction monitoring, proximity extension assays and turbidimetric immunoassay) to quantify precisely 207 serum immune-related proteins in three groups of Crohn's disease patients presenting: 1) no endoscopic lesions (endoscopic remission) (n = 23); 2) isolated ileal ulcers (n = 17); 3) isolated colonic ulcers (n = 16). We found distinct serum proteome signatures associated with ileal and colonic ulcers. Our findings could foster the development of biomarkers and treatments tailored to Crohn's disease location.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
Pierre, Nicolas  ;  Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Huynh-Thu, Vân Anh ;  Université de Liège - ULiège > Département d'électricité, électronique et informatique (Institut Montefiore) > Algorithmique des systèmes en interaction avec le monde physique
Baiwir, Dominique  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
VIEUJEAN, Sophie  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de gastroentérologie, hépatologie, onco. digestive
Bequet, Emeline ;  Université de Liège - ULiège > Département des sciences cliniques > Médecine générale
Reenaers, Catherine ;  Université de Liège - ULiège > Département des sciences cliniques
Van Kemseke, Catherine ;  Université de Liège - ULiège > Département des sciences cliniques
Salée, Catherine ;  Université de Liège - ULiège > GIGA > GIGA I3 - Translational gastroenterology
Massot, Charlotte ;  Université de Liège - ULiège > GIGA > GIGA I3 - Translational gastroenterology
Fleron, Maximilien ;  Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique inorganique
Mazzucchelli, Gabriel  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Trzpiot, Lisette ;  Université de Liège - ULiège > Département de chimie (sciences) > Center for Analytical Research and Technology (CART)
Eppe, Gauthier  ;  Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
De Pauw, Edwin  ;  Université de Liège - ULiège > Département de chimie (sciences)
Louis, Edouard  ;  Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie
Meuwis, Marie-Alice  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Translational gastroenterology
More authors (6 more) Less
Language :
English
Title :
Serum proteome signatures associated with ileal and colonic ulcers in Crohn's disease.
Publication date :
17 May 2024
Journal title :
Journal of Proteomics
ISSN :
1874-3919
eISSN :
1876-7737
Publisher :
Elsevier BV, Netherlands
Pages :
105199
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 May 2024

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