extraction r mass spectrometry r metabolomics r Trypanosoma brucei Fall; F.; Desmet; L.; Mamede; Schioppa; de Tullio; P.; Frédérich; M.; Govaerts; B.; & Quetin-Leclercq; J. (2024). Comparison of three widely employed extraction methods for metabolomic analysis of Trypanosoma brucei. Current Protocols; 4
Abstract :
[en] Trypanosoma brucei (Tb) is the causative agent of human African trypanosomiasis (HAT), also known as sleeping sickness, which can be fatal if left untreated. An understanding of the parasite's cellular metabolism is vital for the discovery of new antitrypanosomal drugs and for disease eradication. Metabolomics can be used to analyze numerous metabolic pathways described as essential to Tb. brucei but has some limitations linked to the metabolites' physicochemical properties and the extraction process. To develop an optimized method for extracting and analyzing Tb. brucei metabolites, we tested the three most commonly used extraction methods, analyzed the extracts by hydrophilic interaction liquid chromatography high-resolution mass spectrometry (HILIC LC-HRMS), and further evaluated the results using quantitative criteria including the number, intensity, reproducibility, and variability of features, as well as qualitative criteria such as the specific coverage of relevant metabolites. Here, we present the resulting protocols for untargeted metabolomic analysis of Tb. brucei using (HILIC LC-HRMS).
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Fall, Fanta; Pharmacognosy Research Group, Louvain Drug Research Institute (LDRI), Université Catholique de Louvain (UCLouvain), Brussels, Belgium
Desmet, Lieven; Institute of Statistics, Biostatistics and Actuarial Sciences, ISBA/LIDAM), UCLouvain, Louvain-la-Neuve, Belgium
Mamede, Lúcia; Laboratory of Pharmacognosy, Center for Interdisciplinary Research on Medicines (CIRM), Center for Interdisciplinary Research on Medicines (CIRM), University of Liège ; University of Liège, Belgium
Schioppa, Laura; Pharmacognosy Research Group, Louvain Drug Research Institute (LDRI), Université Catholique de Louvain (UCLouvain), Brussels, Belgium
De Tullio, Pascal ; Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Govaerts, Bernadette; Institute of Statistics, Biostatistics and Actuarial Sciences, ISBA/LIDAM), UCLouvain, Louvain-la-Neuve, Belgium
Quetin-Leclercq, Joëlle; Pharmacognosy Research Group, Louvain Drug Research Institute (LDRI), Université Catholique de Louvain (UCLouvain), Brussels, Belgium
Language :
English
Title :
Comparison of Three Widely Employed Extraction Methods for Metabolomic Analysis of Trypanosoma brucei
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