Article (Scientific journals)
Effect of monopropylene glycol and gamma irradiation on Yarrowia lipolytica lipase stabilization
Alloué, Wazé Aimée Mireille; Destain, Jacqueline; Ongena, Marc et al.
2008In Preparative Biochemistry and Biotechnology, 38 (3), p. 217-228
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Keywords :
enzymatic activity; formulation; irradiation; monopropylene glycol; protease inhibitors; Yarrowia lipolytica lipase
Abstract :
[en] This work investigated the effects of monopropylene glycol, protease inhibitor, and gamma irradiation on Yarrowia lipolytica lipase stability during storage. Enzyme liquid stabilization was achieved by addition of monopropylene glycol (MPG) at respective concentrations of 50, 75, and 90%, the protease inhibitors (P2714 and P8215) at 0.1%, and the gamma irradiation with 10kGy, 15kGy, and 25kGy doses. The results showed that monopropylene glycol limited the microorganism growth and decreased the enzymatic activity at high concentration (up to 50%), at two temperatures (20 and 4 degrees C). Enzyme stored at 20 degrees C lost its activity by 80% after two months. This loss was attributed to the protease's effect. At this temperature, the protease's activities have been limited by the specific inhibitors. The gamma irradiations improve microbial safety of liquid enzyme.
Disciplines :
Microbiology
Author, co-author :
Alloué, Wazé Aimée Mireille
Destain, Jacqueline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Ongena, Marc ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Blecker, Christophe ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles - Gembloux Agro-Bio Tech
Language :
English
Title :
Effect of monopropylene glycol and gamma irradiation on Yarrowia lipolytica lipase stabilization
Publication date :
2008
Journal title :
Preparative Biochemistry and Biotechnology
ISSN :
1082-6068
eISSN :
1532-2297
Publisher :
Taylor & Francis Ltd
Volume :
38
Issue :
3
Pages :
217-228
217-228
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 03 December 2009

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