Reference : Effect of monopropylene glycol and gamma irradiation on Yarrowia lipolytica lipase st...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/31448
Effect of monopropylene glycol and gamma irradiation on Yarrowia lipolytica lipase stabilization
English
Alloué, Wazé Aimée Mireille [> > > >]
Destain, Jacqueline mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Ongena, Marc mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Blecker, Christophe mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles - Gembloux Agro-Bio Tech >]
2008
Preparative Biochemistry and Biotechnology
Taylor & Francis Ltd
38
3
217-228
Yes (verified by ORBi)
International
1082-6068
1532-2297
[en] enzymatic activity ; formulation ; irradiation ; monopropylene glycol ; protease inhibitors ; Yarrowia lipolytica lipase
[en] This work investigated the effects of monopropylene glycol, protease inhibitor, and gamma irradiation on Yarrowia lipolytica lipase stability during storage. Enzyme liquid stabilization was achieved by addition of monopropylene glycol (MPG) at respective concentrations of 50, 75, and 90%, the protease inhibitors (P2714 and P8215) at 0.1%, and the gamma irradiation with 10kGy, 15kGy, and 25kGy doses. The results showed that monopropylene glycol limited the microorganism growth and decreased the enzymatic activity at high concentration (up to 50%), at two temperatures (20 and 4 degrees C). Enzyme stored at 20 degrees C lost its activity by 80% after two months. This loss was attributed to the protease's effect. At this temperature, the protease's activities have been limited by the specific inhibitors. The gamma irradiations improve microbial safety of liquid enzyme.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/31448
also: http://hdl.handle.net/2268/66756
10.1080/10826060802164462

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