Biochemical and Biophysical Research Communications Metabolomics; Malaria; Plasmodium sp.; Mass Spectrometry; Nuclear Magnetic Resonance; Methodology; Cell Biology; Molecular Biology; Biochemistry; Biophysics
Abstract :
[en] Malaria is a parasitic disease that remains a global concern and the subject of many studies. Metabolomics has emerged as an approach to better comprehend complex pathogens and discover possible drug targets, thus giving new insights that can aid in the development of antimalarial therapies. However, there is no standardized method to extract metabolites from in vitro Plasmodium falciparum intraerythrocytic parasites, the stage that causes malaria. Additionally, most methods are developed with either LC-MS or NMR analysis in mind, and have rarely been evaluated with both tools. In this work, three extraction methods frequently found in the literature were reproduced and samples were analyzed through both LC-MS and 1H NMR, and evaluated in order to reveal which is the most repeatable and consistent through an array of different tools, including chemometrics, peak detection and annotation. The most reliable method in this study proved to be a double extraction with methanol and methanol/water (80:20, v/v). Metabolomic studies in the field should move towards standardization of methodologies and the use of both LC-MS and 1H NMR in order to make data more comparable between studies and facilitate the achievement of biologically interpretable information.
Fall, Fanta; Pharmacognosy research group, Louvain Drug Research Institute (LDRI), UCLouvain, Brussels
Schoumacher, Matthieu ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Ledoux, Allison ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Bugli, Céline; Statistical Methodology and Computing Service (SMCS/LIDAM), UCLouvain, Louvain-la-Neuve, Belgium
De Tullio, Pascal ; Université de Liège - ULiège > Département de pharmacie > Chimie pharmaceutique ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Quetin-Leclercq, Joëlle; Pharmacognosy research group, Louvain Drug Research Institute (LDRI), UCLouvain, Brussels
Govaerts, Bernadette; Statistical Methodology and Computing Service (SMCS/LIDAM), UCLouvain, Louvain-la-Neuve, Belgium
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Language :
English
Title :
Comparison of extraction methods in vitro Plasmodium falciparum: A 1 H NMR and LC-MS joined approach
Publication date :
February 2024
Journal title :
Biochemical and Biophysical Research Communications
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