Article (Scientific journals)
Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid.
Francis, Frédéric; Vanhaelen, Nicolas; Haubruge, Eric
2005In Archives of Insect Biochemistry and Physiology, 58 (3), p. 166-74
Peer Reviewed verified by ORBi
 

Files


Full Text
1525.pdf
Publisher postprint (943.69 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Adaptation, Physiological; Analysis of Variance; Animals; Aphids/drug effects/enzymology; Brassica/chemistry; Chromatography, Affinity; Electrophoresis, Polyacrylamide Gel; Glutathione Transferase/metabolism; Iodobenzenes; Kinetics; Plant Extracts/metabolism/pharmacology
Abstract :
[en] Glutathione S-transferases (GST) in insects play an important role in the detoxification of many substances including allelochemicals from plants. Induction of GST activity in Myzus persicae in response to secondary metabolites from Brassica plants was determined using different host plant species and confirmed using artificial diet with pure allelochemicals added. The 2,4-dinitro-1-iodobenzene (DNIB) was found to be a useful substrate for identifying particular GSTs in insects. GSTs from M. persicae were purified using different affinity chromatography columns and related kinetic parameters were calculated. GST isoenzymes were characterised using electrophoretic methods. Although SDS-PAGE results indicated similarity among the purified enzymes from each affinity column, biochemical studies indicated significant differences in kinetic parameters. Finally, the GST pattern of M. persicae was discussed in terms of insect adaptation to the presence of plant secondary substances such as the glucosinolates and the isothiocyanates, from Brassicaceae host plants.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Francis, Frédéric  ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Vanhaelen, Nicolas
Haubruge, Eric  ;  Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur pour le site de Gembloux
Language :
English
Title :
Glutathione S-transferases in the adaptation to plant secondary metabolites in the Myzus persicae aphid.
Publication date :
2005
Journal title :
Archives of Insect Biochemistry and Physiology
ISSN :
0739-4462
eISSN :
1520-6327
Publisher :
Wiley Liss, Inc., New York, United States - New York
Volume :
58
Issue :
3
Pages :
166-74
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 02 December 2009

Statistics


Number of views
103 (6 by ULiège)
Number of downloads
325 (2 by ULiège)

Scopus citations®
 
162
Scopus citations®
without self-citations
159
OpenCitations
 
141

Bibliography


Similar publications



Contact ORBi