Dual-mode detection; Luminescent metal organic framework; NO(2)(–); On-spot visualization; Rh6G@UIO-66-NH(2); NO2–; Rh6G@UIO-66-NH2; Environmental Engineering; Environmental Chemistry; Waste Management and Disposal; Pollution; Health, Toxicology and Mutagenesis
Abstract :
[en] A novel ratiometric fluorescent nanoprobe (Rh6G@UIO-66-NH2) was fabricated for efficient nitrite (NO2-) detection in the present study. When NO2- was introduced, it interacted with the amino groups on the surface of Rh6G@UIO-66-NH2, forming diazonium salts that led to the quenching of blue fluorescence. With this strategy, a good linear relationship between NO2- concentration and the fluorescent intensity ratio of the nanoprobe in the range of 1-100 μM was established, with a detection limit of 0.021 μM. This dual-readout nanosensor was applied to analyze the concentration of NO2- in real meat samples, achieving satisfactory recovery rates of 94.72-104.52%, highlighting the practical potential of this method. Furthermore, a portable Gel/Rh6G@UIO-66-NH2 hydrogel test kit was constructed for on-spot dual-mode detection of NO2-. This kit allows for convenient colorimetric analysis and fluorometric detection when used in conjunction with a smartphone. All the photos taken with the portable kit was converted into digital information using ImageJ software. It provides colorimetric and fluorescent visual detection of NO2- over a range of 0.1-1.5 mM, achieving a direct quantitative tool for NO2- identification. This methodology presents a promising strategy for NO2- detection and expands the application prospects for on-spot monitoring of food safety assessment.
Disciplines :
Food science
Author, co-author :
Deng, Siyang ; Université de Liège - ULiège > TERRA Research Centre ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Liu, Junmei ; Université de Liège - ULiège > TERRA Research Centre
Han, Dong; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yang, Xinting; Research Center for Information Technology, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China, National Engineering Research Center for Information Technology in Agriculture, Beijing 100097, China
Liu, Huan; Research Center for Information Technology, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China, National Engineering Research Center for Information Technology in Agriculture, Beijing 100097, China. Electronic address: liuhuan@nercita.org.cn
Zhang, Chunhui; Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: dr_zch@163.com
Blecker, Christophe ; Université de Liège - ULiège > TERRA Research Centre > Technologie Alimentaire (TA)
Language :
English
Title :
Synchronous fluorescence detection of nitrite in meat products based on dual-emitting dye@MOF and its portable hydrogel test kit.
Beijing Science and Technology Planning Project National Key Research and Development Program of China
Funders :
CSC - China Scholarship Council [CN]
Funding text :
This project was supported by a research grant from the National Key R & D Program of China ( 2021YFD2100103 ), Beijing Science and Technology Planning Project (grant no. Z221100007122010 ). Siyang Deng’s scholarship was sponsored by the China Scholarship Council (CSC).
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