[en] Next-generation sequencing methods are increasingly used to identify eukaryotic, unicellular and multicellular symbiont communities within hosts. In this study, we analyzed the non-specific reads obtained during a metabarcoding survey of the bacterial communities associated to three different tissues collected from 13 wild Mediterranean teleost fish species. In total, 30 eukaryotic genera were identified as putative parasites of teleosts, associated to skin mucus, gills mucus and intestine: 2 ascomycetes, 4 arthropods, 2 cnidarians, 7 nematodes, 10 platyhelminthes, 4 apicomplexans, 1 ciliate as well as one order in dinoflagellates (Syndiniales). These results highlighted that (1) the metabarcoding approach was able to uncover a large spectrum of symbiotic organisms associated to the fish species studied, (2) symbionts not yet identified in several teleost species were putatively present, (3) the parasitic diversity differed markedly across host species and (4) in most cases, the distribution of known parasitic genera within tissues is in accordance with the literature. The current work illustrates the large insights that can be gained by making maximum use of data from a metabarcoding approach.
Scheifler, Mathilde ; Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs ; Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
Ruiz-Rodríguez, Magdalena; Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
Sanchez-Brosseau, Sophie; Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
Magnanou, Elodie; Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
Suzuki, Marcelino T ; Sorbonne Université, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes, LBBM Observatoire Océanologique, Banyuls/Mer, France
West, Nyree; Sorbonne Université, CNRS, Observatoire Océanologique de Banyuls, Banyuls/Mer, France
Duperron, Sébastien; CNRS, Muséum National d'Histoire Naturelle, Molécules de Communication et Adaptation des Micro-organismes, UMR7245 MCAM, Muséum National d'Histoire Naturelle, Paris, France
Desdevises, Yves; Sorbonne Université, CNRS, Biologie Intégrative des Organismes Marins, BIOM, Observatoire Océanologique, Banyuls/Mer, France
Language :
English
Title :
Characterization of ecto- and endoparasite communities of wild Mediterranean teleosts by a metabarcoding approach
Publication date :
2019
Journal title :
PLoS ONE
eISSN :
1932-6203
Publisher :
Public Library of Science, United States
Volume :
14
Issue :
9
Pages :
e0221475
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
This work was funded by Sorbonne Université, programme Emergence 2016 (project SU-16-R-EMR-22-MICROFISH).
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