Article (Scientific journals)
Effect of NaCl and mannitol iso-osmotic stresses on proline and free polyamine levels in embryogenic Fraxinus angustifolia callus
Tonon, Giustino; Kevers, Claire; Faivre-Rampant, Odile et al.
2004In Journal of Plant Physiology, 161 (6), p. 701-708
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Keywords :
drought; salinity; polyamine; proline; putrescine
Abstract :
[en] With the aim to differentiate the ionic and osmotic components of salt stress, short and long-term changes in free polyamines and proline induced by iso-osmotic concentrations of NaCl (0.1 mol/L and 0.2mol/L) and mannitol (0.2mol/L and 0.4mol/L) were determined in Fraxinus angustifolia callus. The peculiarities of the short-term responses were: i) a very early (30 min) and temporary increase in Putrescine (Pu) and Spermine (Spm) as a consequence of salt treatment, and ii) a continuous accumulation of Spermidine (Spd) and Spm in response to mannitol. The changes of Proline (Pro) were quite limited both in the short and in the long term, and generally occurred later than Polyamine (PAs) changes took place, suggesting a regulatory mechanism of PAs metabolism on Pro biosynthesis. In the long-term, no drastic accumulations of Pro or PAs in response to NaCl and mannitol were observed, suggesting that their physiological role is unlikely to be that of osmo-compatible solutes in this plant system. The salt induced a higher callus growth inhibition effect than did mannitol and this inhibition was associated with the reduction of endogenous levels of PAs, especially Pu. However, while a diverging time course was observed under lethal salt concentration (0.2 mol/L NaCl), a high parallelism in the endogenous changes of Pro and Pu was observed under all non-lethal conditions (control - 0.2 and 0.4 mol/L mannitol - 0.1 mol/L NaCl). Therefore the synchronous changes of Pro and Pu can be considered as a physiological trait associated with cell survival. These results indicate a strong metabolic co-ordination between PAs and Pro pathways and suggest that the metabolic fluxes through these pathways start competing only when the stress level is high enough to be lethal for cells.
Disciplines :
Biotechnology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Tonon, Giustino;  University of Bologna > Dipartimento di Culture Arboree
Kevers, Claire ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Faivre-Rampant, Odile;  Université de Liège - ULiège > Siences de la Vie > Hormonologie Végétale
Graziani, Michele;  University of Bologna > Dipartimento di Culture Arboree
Gaspar, Thomas ;  Botanique, radiobotanique & serres expér.
Language :
English
Title :
Effect of NaCl and mannitol iso-osmotic stresses on proline and free polyamine levels in embryogenic Fraxinus angustifolia callus
Publication date :
June 2004
Journal title :
Journal of Plant Physiology
ISSN :
0176-1617
Publisher :
Urban & Fischer Verlag
Volume :
161
Issue :
6
Pages :
701-708
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 28 December 2008

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