Evaluation of Four Multispecies Probiotic Cocktails in a Human Colonic Fermentation Model

Colon microbiota; Gastrointestinal simulation; Gut microflora; Intestinal cell culture; Multi-strain; Probiotics; Molecular Biology; Molecular Medicine; Microbiology

Abstract :

[en] Bacteriotherapy represents an attractive approach for both prophylaxis and treatment of human diseases. However, combining probiotic bacteria in "cocktails" is underexplored, despite its potential as an alternative multi-target therapy. Herein, three-strain probiotic mixtures containing different combinations of Bacillus (Bc.) coagulans [ATB-BCS-042], Levilactobacillus (Lv.) brevis [THT 0303101], Lacticaseibacillus (Lc.) paracasei [THT 031901], Bacillus subtilis subsp. natto [ATB-BSN-049], Enterococcus faecium [ATB-EFM-030], and Bifidobacterium (Bf.) animalis subsp. lactis [THT 010802] were prepared. Four cocktails (PA: Bc. coagulans + Lv. brevis + Lc. paracasei, PB: Bc. subtilis subsp. natto + Lv. brevis + Lc. paracasei, PC: E. faecium + Lv. brevis + Lc. paracasei, PD: Bc. coagulans + Lv. brevis + Bf. animalis subsp. lactis) were tested using a short-term (72 h) simulation of the human colonic microbiota in a final dose of 6 × 109 CFU. All these probiotic mixtures significantly increased butyrate production compared to the parallel control experiment. PA and PB promoted a bifidogenic effect and facilitated lactobacilli colonization. Furthermore, reporter gene assays using the AhR_HT29-Lucia cell line revealed that fermentation supernatants from PA and PB notably induced AhR transactivity. Subsequent examination of the metabolic outputs of PA and PB in intestinal epithelial models using cell culture inserts suggested no significant impact on the transepithelial electrical resistance (TEER). Assessment of the expression of proinflammatory and anti-inflammatory cytokines, as well as AhR-related target genes in the Caco-2 cell monolayers indicated that PB's metabolic output upregulated most of the measured endpoints. This in vitro investigation evaluated the potential impact of four multispecies probiotic mixtures in the human colonic microbiota and identified a promising formulation comprising a combination of Bc. subtilis subsp. natto, Lv. brevis, and Lc. paracasei as a promising formulation for further study.

Disciplines :

Biotechnology
Food science
Microbiology

Author, co-author :

Goya-Jorge, Elizabeth ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire

Gonza Quito, Irma Elizabeth ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)

Bondue, Pauline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA)

Druart, Germain ; Université de Liège - ULiège > Département GxABT > Technologie Alimentaire (TA)

Al-Chihab, Mohamed; Lacto Research Sprl, Rue Herman Méganck 21, 5032, Isnes-Gembloux, Belgium

Boutaleb, Samiha ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA)

Douny, Caroline ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires

Scippo, Marie-Louise ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Analyse des denrées alimentaires

Thonart, Philippe ; Université de Liège - ULiège > Département GxABT > Microbial technologies

Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Gestion de la qualité dans la chaîne alimentaire

Language :

English

Title :

Evaluation of Four Multispecies Probiotic Cocktails in a Human Colonic Fermentation Model

Publication date :

19 September 2023

Journal title :

Probiotics and Antimicrobial Proteins

ISSN :

1867-1306

eISSN :

1867-1314

Publisher :

Springer Science and Business Media LLC, United States

Volume :

Issue :

Peer reviewed :

Peer reviewed

Additional URL :

https://link.springer.com/content/pdf/10.1007/s12602-023-10162-7.pdf

Name of the research project :

Guttransit Project (Convention N 8225, Pôle–N 27), Wagralim, Wallonie, Belgium

Funders :

Gouvernement Wallon [BE]

Funding number :

8225

Available on ORBi :

since 20 September 2023

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43 (10 by ULiège)

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2 (2 by ULiège)

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