Enzymatic hydrolysis; Gelation; Microstructure; Molecular characteristics; Quinoa protein; Rheological property; Formation mechanism; Physicochemical property; Property; Quinoa proteins; Safety problems; Self-assembled peptides; Self-assembly peptide; Synthesised; Food Science; Chemistry (all); Chemical Engineering (all); General Chemical Engineering; General Chemistry
Abstract :
[en] Currently, self-assembly peptides with hydrogel properties that are used in various applications are being chemically synthesized. These peptides not only have safety and environmental problems but are also expensive and cumbersome to prepare. The present study established a convenient and efficient method for producing plant-based peptides with decent gel-forming ability from quinoa proteins. After alkaline protease treatment, hydrogels made with quinoa protein hydrolysis exhibited potent self-assembly capacity, enhanced gel hardness, and improved rheological properties. Moreover, the microstructure results revealed that the quinoa peptide hydrogels had regular, uniform, and interconnected porous structures. These observations were primarily attributed to the hydrogen bonding force and hydrophobic aggregation caused by hydrophobic group exposure. Amino acid and proteomics analysis suggested that the amino acid composition and sequence of quinoa peptides significantly influenced the formation of self-assembled hydrogels. Overall, this study provided a cost-effective approach to improve the gelling ability of quinoa protein and could potentially replace the use of chemically synthesized peptides in various applications, laying the theoretical basis for the development of novel natural plant-based foods.
Disciplines :
Chemistry
Author, co-author :
Fan, Xin ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Quality Evaluation and Nutrition Health of Agro-Products, Ministry of Agriculture and Rural Affairs, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu, China
Guo, Huimin; Key Laboratory of Quality Evaluation and Nutrition Health of Agro-Products, Ministry of Agriculture and Rural Affairs, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China ; Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium
Richel, Aurore ; Université de Liège - ULiège > TERRA Research Centre > Technologie Alimentaire (TA)
Zhang, Lizhen; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, School of Life Science, Shanxi University, Taiyuan, China
Liu, Chenghong ; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China
Qin, Peiyou ; Key Laboratory of Quality Evaluation and Nutrition Health of Agro-Products, Ministry of Agriculture and Rural Affairs, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu, China
Blecker, Christophe ; Université de Liège - ULiège > TERRA Research Centre > Technologie Alimentaire (TA)
Ren, Guixing; Key Laboratory of Quality Evaluation and Nutrition Health of Agro-Products, Ministry of Agriculture and Rural Affairs, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu, China ; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, School of Life Science, Shanxi University, Taiyuan, China
Language :
English
Title :
Preparation, physicochemical properties, and formation mechanism of quinoa self-assembled peptide-based hydrogel
This work was supported by the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (Grant No. CAAS-ASTIP-2022-ICS), Shanghai Pujiang Program (22PJD066), Shanghai “Science and Technology Innovation Action Plan” (22015810100), a postgraduate international exchange grant from the Chinese Academy of Agricultural Sciences and the China Scholarship Council ( CSC ). The authors would also like to thank Nicolas Jacquet, Lynn Doran and Marjorie Servais from the Teaching and Research Center, Gembloux, Belgium, for their guidance in this study.
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