[en] The isolation of multicellular complexes of thymocytes and different types of thymic stromal cells from the mouse thymus is described. Isolated complexes were examined by light microscopy. Stromal cells, binding thymocytes at their surface, were identified using electron microscopy, and three types of epithelial cells, macrophages and interdigitating-like cells (IDC-like cells) were distinguished from their morphological characteristics. The epithelial cell types correspond morphologically to epithelial cells present in situ in various thymic regions. The type of thymocyte-contact with epithelial cells, macrophages and IDC-like cells indicated that the formation of multicellular complexes is common.
FRSM - Fonds de la Recherche Scientifique Médicale [BE] CAC - Centre anticancéreux près l'Université de Liège asbl [BE] PAN - Polish Academy of Sciences [PL]
Brelińska R. (1981) Morphological types of non-lymphoid cells in the rat thymus. Folia Histochem Cytochem 19:46-47.
Brelińska R., Kaczmarek E., Warchoł J.B., Jaroszewski J. (1985) Distribution of different cell types within the rat thymus in the neonatal period of life. Cell and Tissue Research 240:473-478.
Duijvestijn A.M., Hoefsmit E.C.M. (1981) The micro-environment of T lymphocyte maturation. Cell Tissue Res 218:279-292.
van Ewijk W. (1984) Immunohistology of lymphoid and non-lymphoid cells in the thymus in relation to T lymphocyte differentiation. Am J Anat 170:311-330.
Farr A.G., Nakane P.K. (1983) Cells bearing Ia antigens in the murine thymus. An ultrastructural study. Am J Pathol 111:88-97.
Fink P.J., Weissman I.L., Kaplan H.S., Kyewski B.A. (1984) The immunocompetence of murine stromal cell-associated thymocytes. J Immunol 132:2266-2272.
von Gaudecker B., Schmale E-M (1974) Similarities between Hassall's corpuscles of the human thymus and the epidermis. An investigation by electron microscopy and histochemistry. Cell Tissue Res 151:347-368.
van Haelst U.J.G. (1967) Light and electron microscopic study of the normal and pathologic thymus of the rat. I. The normal thymus. Z Zellforsch 77:534-553.
Hoshino T. (1963) Electron microscopic studies on the epithelial reticular cells of the mouse thymus. Z Zellforsch 59:513-529.
Houben-Defresne M-P, Boniver J. (1983) Thymic nurse cells account for thymus dependency of preleukemic cells in mice after inoculation of radiation leukemia virus. Leukemia Research 7:575-579.
Kaiserling E., Stein H., Müller-Hermelink H.K. (1974) Interdigitating reticulum cells in the human thymus. Cell Tissue Res 155:47-55.
Kyewski B.A., Kaplan H.S. (1982) Lymphoepithelial interactions in the mouse thymus: phenotypic and kinetic studies on thymic nurse cells. J Immunol 128:2287-2294.
Kyewski B.A., Rouse R.V., Kaplan H.S. (1982) Thymocyte rosettes: multicellular complexes of lymphocytes and bone marrow-derived stromal cells in the mouse thymus. Proc Natl Acad Sci USA 79:5646-5650.
Lipsky P.E., Rosenthal A.S. (1973) Macrophage-lymphocyte interaction. I. Characteristics of the antigen independent-binding of guinea pig thymocytes and lymphocytes to syngeneic macrophages. J Exp Med 138:900-924.
Loor F. (1979) Mouse thymus reticulo-epithelial cells in vitro: isolation, cultivation and preliminary characterization. Immunology 37:157-177.
Lopez L.R., Vatter A.E., Talmage D.W. (1977) The requirement of viable thymocytes for species-specific attachment to and release from macrophages. J Immunol 119:1668-1673.
Mandel T. (1970) Differentiation of epithelial cells in the mouse thymus. Z Zellforsch 106:498-515.
Warchoł J.B., Brelińska R. (1982) Studies on Langerhans' cells in the rat thymus. Verh Anat Ges 76:325-326.
Wekerle H., Katelsen U.P., Ernst M. (1980) Thymic nurse cells. Lymphoepithelial complexes in murine thymuses: morphological and serological characterization. J Exp Med 151:925-944.
Wijngaert F.P., Rademakers L.H.P.M., Schuurman H.J., de Weger R.A., Kater L. (1983) Identification and in situ localization of the “thymic nurse cell” in man. J Immunol 130:2348-2351.
Zinkernagel R.M., Callahan G.N., Althage A., Cooper S., Klein P.A., Klein J. (1978) On the thymus in the differentiation of “H-2-self recognition” by T cells. Evidence for dual recognition?. J Exp Med 147:882-896.
