Article (Scientific journals)
IL-4 induces CD22 expression to restrain the effector program of self-reactive virtual memory T cells
Dewals, Benjamin G; Yang, Bin; Sanchez, Guillem Sanchez et al.
2023In Research Square
 

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Abstract :
[en] Abstract Parasitic helminths induce the production of interleukin (IL)-4 which causes the expansion of virtual memory CD8+ T cells (Tvm), a cell subset contributing to the control of viral coinfection. However, the mechanisms regulating IL-4-dependent Tvm activation and expansion during worm infection remain ill defined. We used single-cell RNA sequencing of CD8+ T cells to investigate IL-4-dependent Tvm responses upon helminth infection in mice. Gene signature analysis of CD8+ T cells identified a cell cluster marked by CD22, a canonical regulator of B cell activation, as a specific and selective surface marker of IL-4-induced Tvm cells. CD22+ Tvm were enriched for IFN-γ and granzyme A and retained a diverse TCR repertoire, while enriched in CDR3 sequences with features of self-reactivity. Deletion of CD22 expression in CD8+ T cells enhanced Tvm responses to helminth infection, indicating that this inhibitory receptor modulates Tvm responses. Thus, helminth-induced IL-4 drives the expansion and activation of self-reactive Tvm in the periphery that is counter-inhibited by CD22.
Disciplines :
Life sciences: Multidisciplinary, general & others
Author, co-author :
Dewals, Benjamin G  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
Yang, Bin  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Sanchez, Guillem Sanchez ;  Université Libre de Bruxelles
Lavergne, Arnaud ;  Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) > Génomique animale
Piedfort, Ophélie ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Peng, Garrie;  McGill University
Aguirre, Ariel Madrigal;  McGill University
Petrellis, Georgios  ;  Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
Katsandegwaza, Brunette  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie vétérinaire
Isterdael, Gert Van ;  VIB-UGent
Duyse, Julie Van;  VIB UGent Center for Inflammation Research
Bai, Qiang  ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) ; MedXCell
Marichal, Thomas  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology ; Liège University
Machiels, Bénédicte  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Nitschke, Lars;  Friedrich-Alexander-Universität Erlangen-Nürnberg
Najafabadi, Hamed;  McGill University
King, Irah ;  McGill University Health Centre
Vermijlen, David ;  Department of Pharmacotherapy and Pharmaceutics, Université Libre de Bruxelles (ULB)
More authors (8 more) Less
Language :
English
Title :
IL-4 induces CD22 expression to restrain the effector program of self-reactive virtual memory T cells
Publication date :
06 April 2023
Journal title :
Research Square
Publisher :
Research Square Platform LLC
Available on ORBi :
since 23 June 2023

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