Antigens, Ly; Gold; Animals; Dendritic Cells/immunology; Female; Immunohistochemistry; Macrophages/immunology; Male; Mice; Mice, Inbred C57BL; T-Lymphocytes/classification; T-Lymphocytes/immunology; Thymus Gland/cytology; Thymus Gland/immunology; Anatomy; Agricultural and Biological Sciences (all); General Agricultural and Biological Sciences; General Medicine
Abstract :
[en] Thymocyte subpopulations that are associated with macrophages and dendritic cells of the thymus in vivo were isolated from the thymuses of C57B1/6 mice, and their Lyt phenotypes were analyzed. Electron-microscopic examination of immunogold-labeled cells revealed that the thymic complexes formed by macrophages mainly contained Lyt-2-positive thymocytes, while Lyt-1-positive thymocytes were more frequently associated with dendritic cells. The characteristic distributions of Lyt antigens on the surface of thymocytes in regions of reciprocal contact with macrophages (Lyt-2-positive cells) and dendritic cells (Lyt-1-positive cells) suggest that these antigens play a role in specific interactions between thymocytes and stroma cells.
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