Article (Scientific journals)
MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation.
Vanneste, Domien; Bai, Qiang; Hasan, Shakir et al.
2023In Nature Immunology, 24 (5), p. 827 - 840
Peer Reviewed verified by ORBi
 

Files


Full Text
s41590-023-01468-3 (1).pdf
Author postprint (12.58 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Mafb protein, mouse; MafB Transcription Factor; Animals; Mice; Cell Differentiation; Lung; Cell Proliferation; MafB Transcription Factor/genetics; Monocytes; Macrophages; Immunology and Allergy; Immunology
Abstract :
[en] Resident tissue macrophages (RTMs) are differentiated immune cells that populate distinct niches and exert important tissue-supportive functions. RTM maintenance is thought to rely either on differentiation from monocytes or on RTM self-renewal. Here, we used a mouse model of inducible lung interstitial macrophage (IM) niche depletion and refilling to investigate the development of IMs in vivo. Using time-course single-cell RNA-sequencing analyses, bone marrow chimeras and gene targeting, we found that engrafted Ly6C+ classical monocytes proliferated locally in a Csf1 receptor-dependent manner before differentiating into IMs. The transition from monocyte proliferation toward IM subset specification was controlled by the transcription factor MafB, while c-Maf specifically regulated the identity of the CD206+ IM subset. Our data provide evidence that, in the mononuclear phagocyte system, the ability to proliferate is not merely restricted to myeloid progenitor cells and mature RTMs but is also a tightly regulated capability of monocytes developing into RTMs in vivo.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Vanneste, Domien  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology
Bai, Qiang  ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)
Hasan, Shakir  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology ; Department of Immunology, University of Toronto, Toronto, Canada
Peng, Wen ;  Université de Liège - ULiège > GIGA
Pirottin, Dimitri ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)
Schyns, Joey ;  Université de Liège - ULiège > GIGA > GIGA I3 - Cellular and Molecular Immunology
Maréchal, Pauline ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF)
Ruscitti, Cecilia ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology
Meunier, Margot ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology
Liu, Zhaoyuan;  Shanghai Institute of Immunology, Shanghai JiaoTong University School of Medicine, Shanghai, China
Legrand, Céline ;  Université de Liège - ULiège > GIGA > GIGA I3 - Cellular and Molecular Immunology
Fievez, Laurence ;  Université de Liège - ULiège > Département des sciences fonctionnelles (DSF) > Biochimie et biologie moléculaire
Ginhoux, Florent ;  Shanghai Institute of Immunology, Shanghai JiaoTong University School of Medicine, Shanghai, China ; Inserm U1015, Gustave Roussy, Bâtiment de Médecine Moléculaire, Villejuif, France ; Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore ; Translational Immunology Institute, SingHealth Duke-NUS Academic Medical Centre, Singapore, Singapore
Radermecker, Coraline ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology
Bureau, Fabrice ;  Université de Liège - ULiège > GIGA > GIGA Inflammation, Infection & Immunity
Marichal, Thomas  ;  Université de Liège - ULiège > GIGA > GIGA I3 - Immunophysiology
More authors (6 more) Less
Language :
English
Title :
MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation.
Publication date :
March 2023
Journal title :
Nature Immunology
ISSN :
1529-2908
eISSN :
1529-2916
Publisher :
Nature Research, United States
Volume :
24
Issue :
5
Pages :
827 - 840
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
We thank all members of the Immunophysiology and Cellular and Molecular Immunology laboratories (GIGA Institute, Liège, Belgium) for discussions; S. Ormenese, R. Stefan, A. Hego, G. Lefevre and C. Vanwinge from the GIGA In Vitro Imaging Platform; P. Drion, G. Lambert, L. B. Remy and all staff members from the GIGA Mouse facility and Transgenics Platform; W. Coppieters, L. Karim, M. Deckers, A. Mayer, A. Lavergne and members from the GIGA Genomics Platform; and R. Fares, I. Sbai and A. Lio for their excellent administrative support. T.M. acknowledges support from the F.R.S-FNRS (Incentive Grant for Scientific Research), from the FRFS-Welbio and from the Acteria Foundation. T.M. is supported by a Research Project Grant of the F.R.S.-FNRS, by a FRFS-Welbio Advanced Grant (WELBIO-CR-2022A-10), by an ERC Starting Grant (ERC StG 2018 IM-ID: 801823), by the Baillet Latour Fund, by an ‘Action de Recherche Concertée de la Fédération Wallonie-Bruxelles de Belgique’ and by the Léon Fredericq Foundation; F.B. acknowledges support from the FRFS-Welbio and is supported by an Excellence of Science (EOS) program from the F.R.S.-FNRS and FWO; S.H. received a postdoctoral fellowship from Wallonie-Bruxelles International (WBI); D.V. and C. Ruscitti are research fellows of the F.R.S.-FNRS; W.P. is supported by a fellowship of the China Scholarship Council (CSC); M.M. is a research fellow of the FRIA – FNRS. C. Radermecker is a ‘Chargé de Recherches’ of the F.R.S.-FNRS.
Available on ORBi :
since 22 May 2023

Statistics


Number of views
71 (17 by ULiège)
Number of downloads
29 (7 by ULiège)

Scopus citations®
 
4
Scopus citations®
without self-citations
4
OpenCitations
 
0

Bibliography


Similar publications



Contact ORBi