Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues.

Cancer; Formalin-fixed and paraffin-embedded tissues; Histopathology; Laser microdissection; Microproteomics; Proteins; Formaldehyde; Formaldehyde/chemistry; Humans; Laser Capture Microdissection/methods; Paraffin Embedding/methods; Proteins/analysis; Proteins/isolation & purification; Proteomics/methods; Tissue Fixation/methods; Laser Capture Microdissection; Paraffin Embedding; Proteomics; Tissue Fixation; Molecular Biology; Genetics

Abstract :

[en] Laser microdissection-based proteomics on formalin-fixed and paraffin-embedded tissues is usually performed from relatively large tissue areas or pools of multiple tissue pieces. However, several molecular pathology studies require working on very limited amounts of tissue. This is for example the case when very early cancer lesions have to be handled. Hereby, we present a method for the processing of very small pieces of formalin-fixed and paraffin-embedded tissues for proteomic purposes. This approach is designed in order to avoid sample loss during technical processing and to optimize the digestion of tissue areas containing as little as 2700 cells.

Disciplines :

Life sciences: Multidisciplinary, general & others

Author, co-author :

Longuespée, Rémi ; Université de Liège - ULiège > Département de chimie (sciences) > Center for Analytical Research and Technology (CART) ; Institute of Pathology, University of Heidelberg, Heidelberg, Germany. Remi.Longuespee@med.uni-heidelberg.de

Baiwir, Dominique ; Université de Liège - ULiège > Unités de recherche interfacultaires > GIGA-Technology platforms : Plate-forme protéomique

Mazzucchelli, Gabriel ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Smargiasso, Nicolas ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences)

Language :

English

Title :

Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues.

Publication date :

2018

Journal title :

Methods in Molecular Biology

ISSN :

1064-3745

eISSN :

1940-6029

Publisher :

Humana Press Inc., United States

Volume :

1723

Pages :

19 - 31

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://link.springer.com/content/pdf/10.1007/978-1-4939-7558-7_2

Available on ORBi :

since 01 March 2023

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Bibliography

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