Colon cancer; Gastrointestinal digestion; Microbial dysbiosis; Quinoa protein hydrolysate; Short chain fatty acids; Azoxymethane; Dextran Sulfate; Peptides; Animals; Mice; Azoxymethane/adverse effects; Peptides/pharmacology; Disease Models, Animal; Mice, Inbred C57BL; Colorectal Neoplasms/chemically induced; Colorectal Neoplasms/drug therapy; Colorectal Neoplasms/metabolism; Chenopodium quinoa; Gastrointestinal Microbiome; Probiotics; Colitis/chemically induced; Dextran sulfate sodium; Gut microbiota; Protein hydrolysate; Quinoa proteins; Short chain fatty acid; Shorter chains; Colitis; Colorectal Neoplasms; Analytical Chemistry; Food Science; General Medicine
Abstract :
[en] Quinoa protein hydrolysate has been previously reported to exert anti-cancer effects in cultured colon cancer cells. Here, we investigated the effect of quinoa protein and its hydrolysate on an azoxymethane/dextran sulfate sodium (AOM/DSS)-induced mouse model of colorectal cancer (CRC) and examined its underlying mechanism using gut microbiota analysis and short chain fatty acids (SCFAs) production analysis. Our results showed that quinoa protein or its hydrolysate mitigated the clinical symptoms of CRC and increased SCFAs contents in colon tissues. Moreover, administration of quinoa protein or its hydrolysate partially alleviated gut microbiota dysbiosis in CRC mice by decreasing the abundance of pathogenic bacteria and increasing the abundance of probiotics. Additionally, PICRUSt analysis revealed that the functional profile of gut microbiota in the quinoa protein treated groups was more similar to that of the control group. These findings indicated that the modulation of gut microbiota by quinoa protein diet intervention may ameliorate AOM/DSS-induced CRC.
Disciplines :
Food science
Author, co-author :
Fan, Xin ; Université de Liège - ULiège > TERRA Research Centre
Guo, Huimin; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China, Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium
Teng, Cong; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Yang, Xiushi; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Qin, Peiyou; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Richel, Aurore ; Université de Liège - ULiège > TERRA Research Centre > Smart Technologies for Food and Biobased Products (SMARTECH)
Zhang, Lizhen; School of Life Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China. Electronic address: lizhen@sxu.edu.cn
Blecker, Christophe ; Université de Liège - ULiège > TERRA Research Centre > Smart Technologies for Food and Biobased Products (SMARTECH)
Ren, Guixing; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China, School of Life Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China. Electronic address: renguixing@caas.cn
Language :
English
Title :
Supplementation of quinoa peptides alleviates colorectal cancer and restores gut microbiota in AOM/DSS-treated mice.
The Agricultural Science and Technology Innovation Program
Funders :
CAAS - Chinese Academy of Agricultural Sciences
Funding text :
This work was supported by grants from the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (Grant No. CAAS-ASTIP-2017-ICS) and a postgraduate international exchange grant from the Chinese Academy of Agricultural Sciences .
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