Article (Scientific journals)
Utilization of methyloleate in production of microbial lipase
Destain, Jacqueline; Fickers, Patrick; Weekers, F. et al.
2005In Applied Biochemistry and Biotechnology, 121 (Spring), p. 269-277
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Keywords :
Yarrowia lipolytica; lipase; bioreactor; methyloleate; olive oil; oleic acid
Abstract :
[en] In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Our results demonstrate that methyloleate, a cheap hydrophobic compound, could efficiently substitute olive oil as the inducer and carbon source for lipase production. A new process of lipase production was developed yielding a twofold increase in the level of production compared with the levels in previous reports.
Disciplines :
Biochemistry, biophysics & molecular biology
Biotechnology
Microbiology
Author, co-author :
Destain, Jacqueline ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Fickers, Patrick ;  Université Libre de Bruxelles - ULB > Centre d'ingénierie des protéines
Weekers, F.
Moreau, Benoit
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Utilization of methyloleate in production of microbial lipase
Publication date :
2005
Journal title :
Applied Biochemistry and Biotechnology
ISSN :
0273-2289
eISSN :
1559-0291
Publisher :
Humana Press Inc, Totowa, United States - New Jersey
Volume :
121
Issue :
Spring
Pages :
269-277
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 November 2009

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