Reference : Overproduction of lipase by Yarrowia lipolytica mutants
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biotechnology
Overproduction of lipase by Yarrowia lipolytica mutants
Fickers, Patrick mailto [Université Libre de Bruxelles - ULB > > Centre d'ingénierie des protéines > > >]
Nicaud, J. M. [> > > >]
Destain, Jacqueline mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Thonart, Philippe mailto [Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Applied Microbiology & Biotechnology
Yes (verified by ORBi)
New York
[en] lipase ; Yarrowia lipolytica
[en] Non-genetically modified mutants with increased capacities of extracellular lipase production were obtained from Yarrowia lipolytica strain CBS6303 by chemical mutagenesis. Of the 400 mutants isolated, LgX64.81 had the highest potential for the development of an industrial lipase production process. This mutant exhibits lipase production uncoupled from catabolite repression by glucose, and a 10-fold increased productivity upon addition of oleic acid. Using a LIP2-LacZ reporter gene, we demonstrate that the mutant phenotype originates from a trans-acting mutation. The glucose uptake capacity of LgX64.81 is reduced 2.5-fold compared to the wild-type-strain, and it exhibits high lipase production on glucose medium. A trans-acting mutation in a gene involved in glucose transport could thus explain this mutant phenotype.

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