fatty acids; boar taint; gas chromatography; GC×GC–TOFMS; Lipidomics
Abstract :
[en] Mass spectrometry (MS)-based techniques, including liquid chromatography coupling, shotgun lipidomics, MS imaging, and ion mobility, are widely used to analyze lipids. However, with enhanced separation capacity and an optimized chemical derivatization approach, comprehensive two-dimensional gas chromatography (GC×GC) can be a powerful tool to investigate some groups of small lipids in the framework of lipidomics. This study describes the optimization of a dedicated two-stage derivatization and extraction process to analyze different saturated and unsaturated fatty acids in plasma by two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC–TOFMS) using a full factorial design. The optimized condition has a composite desirability of 0.9159. This optimized sample preparation and chromatographic condition were implemented to differentiate between positive (BT) and negative (UT) boar-tainted pigs based on fatty acid profiling in pig serum using GC×GC–TOFMS. A chemometric screening, including unsupervised (PCA, HCA) and supervised analysis (PLS–DA), as well as univariate analysis (volcano plot), was performed. The results suggested that the concentration of PUFA ω-6 and cholesterol derivatives were significantly increased in BT pigs, whereas SFA and PUFA ω-3 concentrations were increased in UT pigs. The metabolic pathway and quantitative enrichment analysis suggest the significant involvement of linolenic acid metabolism.
Disciplines :
Chemistry
Author, co-author :
Bhatt, Kinjal ; Université de Liège - ULiège > Molecular Systems (MolSys)
Dejong, Thibaut ; Université de Liège - ULiège > Molecular Systems (MolSys)
Dubois, Lena ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Markey, Alice ; Université de Liège - ULiège > TERRA Research Centre
Gengler, Nicolas ; Université de Liège - ULiège > TERRA Research Centre > Ingénierie des productions animales et nutrition
Wavreille, José ; Université de Liège - ULiège > Département GxABT
Stefanuto, Pierre-Hugues ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Focant, Jean-François ; Université de Liège - ULiège > Département de chimie (sciences) > Chimie analytique, organique et biologique
Language :
English
Title :
Lipid Serum Profiling of Boar-Tainted and Untainted Pigs Using GC×GC–TOFMS: An Exploratory Study
Publication date :
15 November 2022
Journal title :
Metabolites
eISSN :
2218-1989
Publisher :
MDPI AG
Special issue title :
Advances in Metabolic Profiling of Biological Samples
FWO - Fonds Wetenschappelijk Onderzoek Vlaanderen [BE] SPW - Public Service of Wallonia [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Funding number :
EOS Grant 30897864; NoWallOdor project (Grant agreements D31-1396 and D65-1430)
Funding text :
This research was funded by the FWO/FNRS Belgium EOS Grant 30897864, “Chemical Information Mining in a Complex World”. The authors acknowledge the support of the Walloon Government (Service Public de Wallonie, Namur, Belgium) through the NoWallOdor project (Grant agreements D31-1396 and D65-1430).
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