NF-kappa B2/p100 induces Bcl-2 expression

[en] The NF-kappaB2/p100 and bcl-3 genes are involved in chromosomal translocations described in chronic lymphocytic leukemias (CLL) and non-Hodgkin's lymphomas, and nuclear factor kappaB (NF-kappaB) protects cancer cells against apoptosis. Therefore, we investigated whether this transcription factor could modulate the expression of the Bcl-2 antiapoptotic protein. Bcl-2 promoter analysis showed multiple putative NF-kappaB binding sites. Transfection assays of bcl-2 promoter constructs in HCT116 cells showed that NF-kappaB can indeed transactivate bcl-2. We identified a kappaB site located at position -180 that can only be bound and transactivated by p50 or p52 homodimers. As p50 and p52 homodimers are devoid of any transactivating domains, we showed that they can transactivate the bcl-2 promoter through association with Bcl-3. We also observed that stable overexpression of p100 and its processed product p52 can induce endogenous Bcl-2 expression in MCF7AZ breast cancer cells. Finally, we demonstrated that, in breast cancer and leukemic cells ( CLL), high NF-kappaB2/p100 expression was associated with high Bcl-2 expression. Our data suggest that Bcl-2 could be an in vivo target gene for NF-kappaB2/p100.

Research Center/Unit :

Giga-Signal Transduction - ULiège

Disciplines :

Oncology
Hematology

Author, co-author :

Viatour, Patrick ; Université de Liège - ULiège > Chimie médicale

Bentires-Alj, Mohamed

Chariot, Alain ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Deregowski, Valérie

de Leval, Laurence ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques

Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Bours, Vincent ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine

Language :

English

Title :

NF-kappa B2/p100 induces Bcl-2 expression

Publication date :

July 2003

Journal title :

Leukemia

ISSN :

0887-6924

eISSN :

1476-5551

Publisher :

Nature Publishing Group, London, United Kingdom

Volume :

Issue :

Pages :

1349-1356

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
Télévie
FBC
ULg. ARC - Université de Liège. Actions de Recherche Concertées

Available on ORBi :

since 20 December 2008

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Scopus citations^®

150

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149

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128

OpenAlex citations

165

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