Effect of protective compounds on the survival, electrolyte leakage and lipid degradation of freeze-dried Weissella paramesenteroides LC11 during storage.
[en] The effect of cryoprotectants (maltodextrin+glycerol) and cryoprotectants+antioxidant [ascorbic acid and/or butylated hydroxytoluene (BHT)] mixtures on the survival, electrolyte leakage, and lipid degradation of freeze-dried Weissella paramesenteroides LC11 during storage was investigated and compared with that of the control (cells without additives) over a 90-day storage period at 4 or 20 degrees in glass tubes with water activity (a(w)) of 0.23. The survival, electrolyte leakage, and lipid degradation were evaluated through colony counts, electrical conductivity, and thiobarbituric acid reactive substances (TBARS) content, respectively. The fatty acids composition was determined by gas chromatography, in both the total lipid extract and the polar lipid fraction, and compared with that of the control after the 90-day storage period. As the storage proceeded, increases in leakage value and TBARS content, as well as a decrease in viability, were observed. After 90 days of storage, the major fatty acids found in both the total lipid extract and the polar lipid fraction were palmitic (16:0), palmitoleic (16:1), stearic (18:0), oleic (18:1), linoleic (18:2), and linolenic (18:3) acids. The survival, leakage value, TBARS content and 18:2/16:0 or 18:3/16:0 ratio were the greatest for the protected strain held at 4 degrees . Cells with the cryoprotectants+BHT mixture showed the highest percentage of survival and 18:2/16:0 or 18:3/16:0 ratio in both lipid extracts, as well as the lowest leakage value and TBARS content after the 90-day storage period. Drying cells with the cryoprotectants+BHT mixture considerably slowed down polar lipid degradation and loss of membrane integrity, resulting in improved viability during storage.
Wathelet, Bernard ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles - Bio-industries
Language :
English
Title :
Effect of protective compounds on the survival, electrolyte leakage and lipid degradation of freeze-dried Weissella paramesenteroides LC11 during storage.
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Bibliography
Borsos-Matovina, V. and T. J. Blake. 2001. Seed treatment with the antioxidant Ambiol enhances membrane protection in seedlings exposed to drought and low temperatures. Trees 15: 163-167.
Castro, H. P., P. M. Teixeira, and R. Kirby. 1995. Storage of lyophilized cultures of Lactobacillus bulgaricus under different relative humidities and atmospheres. Appl. Microbiol. Biotechnol. 44: 172-176.
Castro, H. P., P. M. Teixeira, and R. Kirby R. 1996. Changes in the cell membrane of Lactobacillus bulgaricus during storage following freeze-drying. Biotechnol. Lett. 18: 99-104.
Champagne, C. P., F. Mondou, Y. Raymond, and D. Roy. 1996. Effect of polymers and storage temperature on the stability of freeze-dried lactic acid bacteria. Food Res. Int. 29: 555-562.
Coulibaly, I., A. A. Yao, G. Lognay, J. Destain, M.-L. Fauconnier, and P. Thonart. 2008. Survival of freeze-dried Leuconostoc mesenteroides and Lactobacillus plantarum related to their cellular fatty acids composition during storage. Appl. Biochem. Biotechnol. 157: 70-84.
Halliwell, B. and S. Chirico. 1993. Lipid peroxidation: Its mechanisms, measurement, and significance. Am. Clin. Nutr. 57: 715s-724s.
Hamoudi, L., J. Goulet, and C. Ratti. 2007. Effect of protective agents on the viability of Geotricum candidum during freeze-drying and storage. Food Microbiol. Food Saf. 72: M45-M49.
Hayashi, T., A. Terao, S. Ueda, and M. Namiki. 1985. Red pigment formation by the reaction of oxidized ascorbic acid and protein in a food model system of low moisture content. Agric. Biol. Chem. 49: 3139-3144.
Heckly, R. J. 1985. Principles of preserving bacteria by freeze-drying. Dev. Ind. Microbiol. 26: 379-395.
Hurst, A. 1977. Bacterial injury: A review. Can. J. Microbiol. 23: 936-944.
Ishibashi, N., I. Tatematsu, S. Shimamura, M. Tomita, and S. Okonogi. 1985. Effect of water activity on the viability of freeze-dried bifidobacteria and lactic acid bacteria, pp. 227-232. I. I. R.-I. I. F. Commission C1, Tokyo, Japan.
Kostinek, M., I. Specht, V. A. Edward, C. Pinto, M. Egounlety, C. Sossa, et al. 2007. Characterisation and biochemical properties of predominant lactic acid bacteria from fermenting cassava for selection as starter cultures. Int. J. Food Microbiol. 114: 342-351.
Kostinek, M., I. Specht, V. A. Edward, U. Schillinger, C. Hertel, W. H. Holzapfel, and C. M. A. P. Franz. 2005. Diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of Gari, a traditional African food. Syst. Appl. Microbiol. 28: 527-540.
Lefèvre, G., M. Beljean-Leymarie, F. Beyerle, D. Bonnefont-Rousselot, J. P. Cristol, P. Thérond, and J. Torreilles. 1998. Evaluation de la peroxydation lipidique par le dosage des substances réagissant avec l'acide thiobarbiturique. Ann. Biol. Clin. 56: 305-319.
Martos, G. I., A. P. Ruiz Holgado, G. Oliver, and G. F. De Valdez. 1999. Use of conductimetry to evaluate Lactobacillus delbrueckii ssp. bulgaricus subjected to freeze-drying. Milchwissenschaft 54: 128-130.
Raharjo, S. and J. N. Sofos. 1993. Methodology for measuring malondialdehyde as a product of lipid peroxidation in muscle tissues: A review. Meat Sci. 35: 145-169.
Raharjo, S., J. N. Sofos, and G. R. Schmidt. 1992. Improved speed, specificity, and limit determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid peroxidation in beef. J. Agric. Food Chem. 40: 2182-2185.
Selmer-Olsen, E., S. E. Birkeland, and T. Sørhaug. 1999. Effect of protective solutes on leakage from and survival of immobilized Lactobacillus subjected to drying, storage and rehydration. J. Appl. Microbiol. 87: 429-437.
St. Angelo, A. J. 1996. Lipid oxidation in foods. Crit. Rev. Food Sci. Nutr. 36: 175-224.
Tarladgis, B. O., A. M. Pearson, and L. R. Jr Dugan. 1964. Chemistry of the 2-thiobarbituric acid test for determination of oxidative rancidity in foods: (II) Formation of the TBA-malonaldehyde complex without acid-heat treatment. J. Sci. Food Agric. 15: 602-607.
Teixeira, P. C., M. H. Castro, F. X. Malcata, and R. M. Kirby. 1995. Survival of Lactobacillus delbrueckii ssp. bulgaricus following spray drying. J. Dairy Sci. 78: 1025-1031.
van Ginkel, G. and A. Sevanian. 1994. Lipid peroxidation-induced membrane structural alterations. Methods Enzymol. 233: 273-288. (Pubitemid 24177551)
Vandana, V., M. S. L. Karuna, P. Vijayalakshmi, and R. B. N. Prasad. 2001. A simple method to enrich phospholipid content in commercial soybean lecithin. J. Am. Oil Chem. Soc. 78: 555-556.
Wang, Y.-C., R.-C. Yu, and C.-C. Chou. 2004. Viability of lactic acid bacteria and bifidobacteria in fermented soymilk after drying, subsequent rehydratation and storage. Int. J. Food Microbiol. 93: 209-217.
Yao, A. A., I. Coulibaly, G. Lognay, M.-L. Fauconnier, and P. Thonart. 2008. Impact of polyunsaturated fatty acid degradation on survival and acidification activity of freeze-dried Weissella paramesenteroides LC11 during storage. Appl. Microbiol. Biotechnol. 79: 1045-1052.
Yao, A. A., F. Bera, C. M. A. P. Franz, W. H. Holzapfel, and P. Thonart. 2008. Survival rate analysis of freeze-dried lactic acid bacteria using the Arrhenius and z-value models. J. Food Prot. 71: 431-434.
Zhao, G. and G. Zhang. 2005. Effect of protective agents, freezing temperature, rehydratation media on viability of malolactic bacteria subjected to freeze-drying. J. Appl. Microbiol. 99: 333-338.
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