[en] This study aimed at clarifying the mechanism by which sweet potato leaf polyphenols (SPLPs) ameliorate ultraviolet (UV) radiation damage, using the BALB/c hairless female mouse model. The moisture and hydroxyproline (HYP) contents of the model mouse skin and the thickness of the epidermis and dermis were determined by staining and histological examination. Anti-oxidative enzyme activities, malondialdehyde (MDA) content, and protein carbonyl content in skin tissue and serum were investigated. Expression of inflammatory markers and mitogen-activated protein kinase signaling pathways were evaluated. Topical caffeic acid at 30 mg kg-1 most strongly inhibited the decrease in skin moisture, HYP content, and the thickening of the epidermis. Topical SPLP at 100 mg kg-1 most significantly inhibited the dermal thickening, increased the activities of the superoxide dismutase, catalase as well as glutathione peroxidase, and decreased the content of serum MDA and protein carbonyls markedly. Furthermore, the topical SPLP suppressed the UV-induced rise in the inflammatory markers MMP-1, TNF-α, and NF-κB, and alleviated phosphorylation levels of the stress-signaling proteins JNK and p38. Thus, topical SPLP provided the best overall protection for mouse skin from UV-induced damage.
Disciplines :
Food science
Author, co-author :
Hong, Jingyang ; Université de Liège - ULiège > TERRA Research Centre ; Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, No. 2 Yuan Ming Yuan West Road, Haidian District, P.O. Box 5109, Beijing 100193, China. mutaihua@126.com
Mu, Taihua ; Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, No. 2 Yuan Ming Yuan West Road, Haidian District, P.O. Box 5109, Beijing 100193, China. mutaihua@126.com
Sun, Hongnan; Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, No. 2 Yuan Ming Yuan West Road, Haidian District, P.O. Box 5109, Beijing 100193, China. mutaihua@126.com
Blecker, Christophe ; Université de Liège - ULiège > Département GxABT > Smart Technologies for Food and Biobased Products (SMARTECH)
Richel, Aurore ; Université de Liège - ULiège > Département GxABT > Smart Technologies for Food and Biobased Products (SMARTECH)
Language :
English
Title :
Photoprotective effects of sweet potato leaf polyphenols and caffeic acid against UV-induced skin-damage in BALB/C nude mice.
National Key Research and Development Program of China Chinese Academy of Agricultural Sciences Agriculture Research System of China
Funding text :
The authors gratefully acknowledge the National Key R&D Program of China (2016YFE0133600), the China Agriculture Research System of MOF and MARA (CARS-10-B21), the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-202X-IFST), and the Central Public-Interest Scientific Institution Basal Research Fund (S2020JBKY-09).
Ferrara F. Pambianchi E. Woodby B. Messano N. Therrien J. P. Pecorelli A. Canella R. Valacchi G. Evaluating the effect of ozone in UV induced skin damage Toxicol. Lett. 2021 338 40 50 10.1016/j.toxlet.2020.11.023 33279629
Rivas M. Calaf G. M. Laroze D. Rojas E. Mendez J. Honeyman J. Araya M. C. Solar ultraviolet A radiation and nonmelanoma skin cancer in Arica, Chile J. Photochem. Photobiol., B 2020 212 112047 10.1016/j.jphotobiol.2020.112047 33045532
Marabini L. Melzi G. Lolli F. Dell'Agli M. Piazza S. Sangiovanni E. Marinovich M. Effects of Vitis vinifera L. leaves extract on UV radiation damage in human keratinocytes (HaCaT) J. Photochem. Photobiol., B 2020 204 111810 10.1016/j.jphotobiol.2020.111810 32028189
Ruuhola T. Nybakken L. Randriamanana T. Lavola A. Julkunen-Tiitto R. Effects of long-term UV-exposure and plant sex on the leaf phenoloxidase activities and phenolic concentrations of Salix myrsinifolia (Salisb.) Plant Physiol. Biochem. 2018 126 55 62 10.1016/j.plaphy.2018.02.025 29501893
Garg C. Sharma H. Garg M. Skin photo-protection with phytochemicals against photo-oxidative stress, photo-carcinogenesis, signal transduction pathways and extracellular matrix remodeling—An overview Ageing Res. Rev. 2020 62 101127 10.1016/j.arr.2020.101127 32721499
Hu S. Zhang X. Chen F. Wang M. Dietary polyphenols as photoprotective agents against UV radiation J. Funct. Foods 2017 30 108 118 10.1016/j.jff.2017.01.009
Farrar M. D. Nicolaou A. Clarke K. A. Mason S. Massey K. A. Dew T. P. Watson R. E. B. Williamson G. Rhodes L. E. A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation1,2 Am. J. Clin. Nutr. 2015 102 608 615 10.3945/ajcn.115.107995 26178731
Hong J. Mu T. Sun H. Richel A. Blecker C. Valorization of the green waste parts from sweet potato (Impoea batatas L.): Nutritional, phytochemical composition, and bioactivity evaluation Food Sci. Nutr. 2020 8 4086 4097 10.1002/fsn3.1675 32884690
Mu T.-H. and Sun H.-N., Sweet Potato Leaf Polyphenols: Preparation, Individual Phenolic Compound Composition and Antioxidant Activity, in Polyphenols in Plants, Elsevier Inc., 2nd edn, 2019, pp. 365-380
Sato Y. Itagaki S. Kurokawa T. Ogura J. Kobayashi M. Hirano T. Sugawara M. Iseki K. In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid Int. J. Pharm. 2011 403 136 138 10.1016/j.ijpharm.2010.09.035 20933071
Kligman L. H. The hairless mouse model for photoaging Clin. Dermatol. 1996 14 183 195 10.1016/0738-081X(95)00154-8 9117985
Calcutt J. J. Anissimov Y. G. Physiologically based mathematical modelling of solute transport within the epidermis and dermis Int. J. Pharm. 2019 569 118547 10.1016/j.ijpharm.2019.118547 31377408
Song H. Meng M. Cheng X. Li B. Wang C. The effect of collagen hydrolysates from silver carp (: Hypophthalmichthys molitrix) skin on UV-induced photoaging in mice: Molecular weight affects skin repair Food Funct. 2017 8 1538 1546 10.1039/C6FO01397J 28266663
Wang J. Qiu H. Xu Y. Gao Y. Tan P. Zhao R. Liu Z. Tang Y. Zhu X. Bao C. Wang H. Lin H. Zhang X. The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: An in vivo study Bioact. Mater. 2022 11 154 165 10.1016/j.bioactmat.2021.10.004 34938920
Liu Y. Su G. Zhou F. Zhang J. Zheng L. Zhao M. Protective Effect of Bovine Elastin Peptides against Photoaging in Mice and Identification of Novel Antiphotoaging Peptides J. Agric. Food Chem. 2018 66 10760 10768 10.1021/acs.jafc.8b04676 30269487
Masaki H. Role of antioxidants in the skin: Anti-aging effects J. Dermatol. Sci. 2010 58 85 90 10.1016/j.jdermsci.2010.03.003 20399614
Zhi Q. Lei L. Li F. Zhao J. Yin R. Ming J. The anthocyanin extracts from purple-fleshed sweet potato exhibited anti-photoaging effects on ultraviolent B-irradiated BALB/c-nu mouse skin J. Funct. Foods 2020 64 103640 10.1016/j.jff.2019.103640
Rabe J. H. Mamelak A. J. McElgunn P. J. S. Morison W. L. Sauder D. N. Photoaging: Mechanisms and repair J. Am. Acad. Dermatol. 2006 55 1 19 10.1016/j.jaad.2005.05.010 16781287
Hou H. Li B. Zhang Z. Xue C. Yu G. Wang J. Bao Y. Bu L. Sun J. Peng Z. Su S. Moisture absorption and retention properties, and activity in alleviating skin photodamage of collagen polypeptide from marine fish skin Food Chem. 2012 135 1432 1439 10.1016/j.foodchem.2012.06.009 22953877
Ye Y. Ji D. You L. Zhou L. Zhao Z. Brennan C. Structural properties and protective effect of Sargassum fusiforme polysaccharides against ultraviolet B radiation in hairless Kun Ming mice J. Funct. Foods 2018 43 8 16 10.1016/j.jff.2018.01.025
Zhong Q. Y. Lin B. Chen Y. T. Huang Y. P. Feng W. P. Wu Y. Long G. H. Zou Y. N. Liu Y. Lin B. Q. Sang N. L. Zhan J. Y. X. Gender differences in UV-induced skin inflammation, skin carcinogenesis and systemic damage Environ. Toxicol. Pharmacol. 2021 81 103512 10.1016/j.etap.2020.103512 33096234
Xu D. Li D. Zhao Z. Wu J. Zhao M. Regulation by walnut protein hydrolysate on the components and structural degradation of photoaged skin in SD rats Food Funct. 2019 10 6792 6802 10.1039/C8FO01833B 31577280
Kremer J. L. Melo G. P. Marinello P. C. Bordini H. P. Rossaneis A. C. Sábio L. R. Cecchini R. Cecchini A. L. Verri W. A. Luiz R. C. Citral prevents UVB-induced skin carcinogenesis in hairless mice J. Photochem. Photobiol., B 2019 198 111565 10.1016/j.jphotobiol.2019.111565 31374350
Zhang L. Zheng Y. Cheng X. Meng M. Luo Y. Li B. The anti-photoaging effect of antioxidant collagen peptides from silver carp (Hypophthalmichthys molitrix) skin is preferable to tea polyphenols and casein peptides Food Funct. 2017 8 1698 1707 10.1039/C6FO01499B 28266664
Koh E. K. Kim J. E. Go J. Song S. H. Sung J. E. Son H. J. Jung Y. J. Kim B. H. Jung Y. S. Hwang D. Y. Protective effects of the antioxidant extract collected from Styela clava tunics on UV radiation induced skin aging in hairless mice Int. J. Mol. Med. 2016 38 1565 1577 10.3892/ijmm.2016.2740 27665714
Zawrotniak M. Bartnicka D. Rapala-Kozik M. UVA and UVB radiation induce the formation of neutrophil extracellular traps by human polymorphonuclear cells J. Photochem. Photobiol., B 2019 196 111511 10.1016/j.jphotobiol.2019.111511 31129510
Liu S. You L. Zhao Y. Chang X. Hawthorn Polyphenol Extract Inhibits UVB-Induced Skin Photoaging by Regulating MMP Expression and Type i Procollagen Production in Mice J. Agric. Food Chem. 2018 66 8537 8546 10.1021/acs.jafc.8b02785 30032605
Kim S. Chung J. H. Berberine prevents UV-induced MMP-1 and reduction of type I procollagen expression in human dermal fibroblasts Phytomedicine 2008 15 749 753 10.1016/j.phymed.2007.11.004 18164189
Vilela F. M. P. Fonseca Y. M. Jabor J. R. Vicentini F. T. Fonseca M. J. V. Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation Eur. J. Pharm. Biopharm. 2012 80 387 392 10.1016/j.ejpb.2011.10.005 22036989
Ho J. C. Chan-Yeung M. Ho S. P. Mak J. C. Ip M. S. Ool G. C. Wong M. P. Tsang K. W. Lam W. K. Disturbance of systemic antioxidant profile in nonsmall cell lung carcinoma Eur. Respir. J. 2007 29 273 278 10.1183/09031936.00000106 17005588
Wu Y. Tian Q. Li L. Khan M. N. Yang X. Zhang Z. Hu X. Chen S. Inhibitory effect of antioxidant peptides derived from Pinctada fucata protein on ultraviolet-induced photoaging in mice J. Funct. Foods 2013 5 527 538 10.1016/j.jff.2013.01.016
Parzonko A. Kiss A. K. Caffeic acid derivatives isolated from Galinsoga parviflora herb protected human dermal fibroblasts from UVA-radiation Phytomedicine 2019 57 215 222 10.1016/j.phymed.2018.12.022 30785017
Khan N. Syed D. N. Pal H. C. Mukhtar H. Afaq F. Pomegranate fruit extract inhibits UVB-induced inflammation and proliferation by modulating NF-κB and MAPK signaling pathways in mouse skin Photochem. Photobiol. 2012 88 1126 1134 10.1111/j.1751-1097.2011.01063.x 22181855
Offord E. A. Gautier J. C. Avanti O. Scaletta C. Runge F. Krämer K. Applegate L. A. Photoprotective potential of lycopene, β-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts Free Radicals Biol. Med. 2002 32 1293 1303 10.1016/S0891-5849(02)00831-6 12057767
Huang C. H. Li H. J. Wu N. L. Hsiao C. Y. Lin C. N. Chang H. H. Hung C. F. Photoprotective effects of cycloheterophyllin against UVA-induced damage and oxidative stress in human dermal fibroblasts PLoS One 2016 11 1 14
Balupillai A. Nagarajan R. P. Ramasamy K. Govindasamy K. Muthusamy G. Caffeic acid prevents UVB radiation induced photocarcinogenesis through regulation of PTEN signaling in human dermal fibroblasts and mouse skin Toxicol. Appl. Pharmacol. 2018 352 87 96 10.1016/j.taap.2018.05.030 29802912
Song H. Meng M. Cheng X. Li B. Wang C. The effect of collagen hydrolysates from silver carp (: Hypophthalmichthys molitrix) skin on UV-induced photoaging in mice: Molecular weight affects skin repair Food Funct. 2017 8 1538 1546 10.1039/C6FO01397J 28266663
Fan J. Zhuang Y. Li B. Effects of collagen and collagen hydrolysate from jellyfish umbrella on histological and immunity changes of mice photoaging Nutrients 2013 5 223 233 10.3390/nu5010223 23344251
Chen T. Hou H. Fan Y. Wang S. Chen Q. Si L. Li B. Protective effect of gelatin peptides from pacific cod skin against photoaging by inhibiting the expression of MMPs via MAPK signaling pathway J. Photochem. Photobiol., B 2016 165 34 41 10.1016/j.jphotobiol.2016.10.015 27768951
Bosch R. Philips N. Suárez-Pérez J. A. Juarranz A. Devmurari A. Chalensouk-Khaosaat J. González S. Mechanisms of photoaging and cutaneous photocarcinogenesis, and photoprotective strategies with phytochemicals Antioxidants 2015 4 248 268 10.3390/antiox4020248 26783703
Popovic N. Pajovic S. B. Stojiljkovic V. Todorovic A. Pejic S. Pavlovic I. Gavrilovic L. Increased activity of hippocampal antioxidant enzymes as an important adaptive phenomenon of the antioxidant defense system in chronically stressed rats Acta Vet. Brno 2017 67 540 550 10.1515/acve-2017-0043
Weber D. Davies M. J. Grune T. Determination of protein carbonyls in plasma, cell extracts, tissue homogenates, isolated proteins: Focus on sample preparation and derivatization conditions Redox Biol. 2015 5 367 380 10.1016/j.redox.2015.06.005 26141921
Pittayapruek P. Meephansan J. Prapapan O. Komine M. Ohtsuki M. Role of matrix metalloproteinases in Photoaging and photocarcinogenesis Int. J. Mol. Sci. 2016 17 868 10.3390/ijms17060868 27271600
Xiao J. Liu B. Zhuang Y. Effects of rambutan (Nephelium lappaceum) peel phenolics and Leu-Ser-Gly-Tyr-Gly-Pro on hairless mice skin photoaging induced by ultraviolet irradiation Food Chem. Toxicol. 2019 129 30 37 10.1016/j.fct.2019.04.020 31009737
Katiyar S. K. Dietary proanthocyanidins inhibit UV radiation-induced skin tumor development through functional activation of the immune system Mol. Nutr. Food Res. 2016 60 1374 1382 10.1002/mnfr.201501026 26991736
de Souza R. O. de Assis Dias Alves G. Aguillera A. L. S. Rogez H. Fonseca M. J. V. Photochemoprotective effect of a fraction of a partially purified extract of Byrsonima crassifolia leaves against UVB-induced oxidative stress in fibroblasts and hairless mice J. Photochem. Photobiol., B 2018 178 53 60 10.1016/j.jphotobiol.2017.10.033 29107927
