antioxidant capacity; functional properties; germinated soybean; protein structure; selenium biofortification; Food Science; Endocrinology, Diabetes and Metabolism; Nutrition and Dietetics
Abstract :
[en] Selenium (Se) biofortification during germination is an efficient method for producing Se-enriched soybean sprouts; however, few studies have investigated Se distribution in different germinated soybean proteins and its effects on protein fractions. Herein, we examined Se distribution and speciation in the dominant proteins 7S and 11S of raw soybean (RS), germinated soybean (GS), and germinated soybean with Se biofortification (GS-Se). The effects of germination and Se treatment on protein structure, functional properties, and antioxidant capacity were also determined. The Se concentration in GS-Se was 79.8-fold higher than that in GS. Selenomethionine and methylselenocysteine were the dominant Se species in GS-Se, accounting for 41.5-80.5 and 19.5-21.2% of the total Se with different concentrations of Se treatment, respectively. Se treatment had no significant effects on amino acids but decreased methionine in 11S. In addition, the α-helix contents decreased as the Se concentration increased; the other structures showed no significant changes. The Se treatment also had no significant effects on the water and oil-holding capacities in protein but increased the foaming capacity and emulsion activity index (EAI) of 7S, but only the EAI of 11S. The Se treatment also significantly increased the antioxidant capacity in 7S but not in 11S. This study indicates that the dominant proteins 7S and 11S have different Se enrichment abilities, and the protein structures, functional properties, and antioxidant capacity of GS can be altered by Se biofortification.
Disciplines :
Food science
Author, co-author :
Huang, Yatao ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Fan, Bei; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Lei, Ningyu; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Xiong, Yangyang; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Liu, Yanfang; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Tong, Litao; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Wang, Fengzhong; Key Laboratory of Agro-Products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China
Maesen, Philippe ; Université de Liège - ULiège > GxABT : Centres attachés > Bureau Environnement et Analyse (GxABT)
Blecker, Christophe ; Université de Liège - ULiège > TERRA Research Centre > Smart Technologies for Food and Biobased Products (SMARTECH)
Language :
English
Title :
Selenium Biofortification of Soybean Sprouts: Effects of Selenium Enrichment on Proteins, Protein Structure, and Functional Properties.
National Key Research and Development Program of China Agriculture Research System of China
Funding text :
This study was supported by the Special National Key Research and Development Plan (2021YFD1600101), the Quality and Safety of Agricultural Products (Risk Assessment) from the Chinese Ministry of Agriculture and Rural Affairs (Grant No. GJFP2019001), and the China Agriculture Research System (CARS-04).
Day L. Proteins from land plants–potential resources for human nutrition and food security. Trends Food Sci Technol. (2013) 32:25–42. 10.1016/j.tifs.2013.05.005
Patil G Mian R Vuong T Pantalone V Song Q Chen P et al. Molecular mapping and genomics of soybean seed protein: a review and perspective for the future. Theor Appl Genet. (2017) 130:1975–91. 10.1007/s00122-017-2955-828801731
Makeri MU Mohamed SA Karim R Ramakrishnan Y Muhammad K. Fractionation, physicochemical, and structural characterization of winged bean seed protein fractions with reference to soybean. Int J Food Prop. (2017) 2017:1–17. 10.1080/10942912.2017.1369101
Sha L Liu S Liu D. Effects of soybean protein isolate on protein structure, batter rheology, and water migration in emulsified sausage. J Food Proces Preserv. (2020) 44:14711. 10.1111/jfpp.14711
Liang Y Chen B Li X Dimler R. Fortification of concentrated milk protein beverages with soy proteins: impact of divalent cations and heating treatment on the physical stability. J Food Process Technol. (2018) 9:735. 10.4172/2157-7110.1000735
Wang C Yin H Zhao Y Zheng Y Xu X Yue J. Optimization of high hydrostatic pressure treatments on soybean protein isolate to improve its functionality and evaluation of its application in yogurt. Foods. (2021) 10:30667. 10.3390/foods1003066733804726
Deng X Liao J Zhao Z Qin Y Liu X. Distribution and speciation of selenium in soybean proteins and its effect on protein structure and functionality. Food Chem. (2021) 370:130982. 10.1016/j.foodchem.2021.13098234537428
Hatfield DL Tsuji PA Carlson BA Gladyshev VN. Selenium and selenocysteine: roles in cancer, health, and development. Trends Biochem Sci. (2014) 39:112–20. 10.1016/j.tibs.2013.12.00724485058
Kipp AP Strohm D Brigelius-Flohe R Schomburg L Bechthold A Leschik-Bonnet E et al. Revised reference values for selenium intake. J Trace Elem Med Biol. (2015) 32:195–9. 10.1016/j.jtemb.2015.07.00526302929
Ingold I Berndt C Schmitt S Doll S Poschmann G Buday K et al. Selenium utilization by GPX4 is required to prevent hydroperoxide-induced ferroptosis. Cell. (2018) 172:409–22. e21. 10.1016/j.cell.2017.11.04829290465
Hu J Zhao Q Cheng X Selomulya C Bai C Zhu X et al. Antioxidant activities of Se-SPI produced from soybean as accumulation and biotransformation reactor of natural selenium. Food Chem. (2014) 146:531–7. 10.1016/j.foodchem.2013.09.08724176378
Zhao X Gao J Hogenkamp A Knippels LM Garssen J Bai J et al. Selenium-enriched soy protein has antioxidant potential via modulation of the NRF2-HO1 signaling pathway. Foods. (2021) 10:2542. 10.3390/foods1011254234828827
Li Y Zhu N Liang X Zheng L Zhang C Li YF et al. A comparative study on the accumulation, translocation and transformation of selenite, selenate, and SeNPs in a hydroponic-plant system. Ecotoxicol Environ Saf. (2020) 189:109955. 10.1016/j.ecoenv.2019.10995531759745
Umysova D Vitova M Douskova I Bisova K Hlavova M Cizkova M et al. Bioaccumulation and toxicity of selenium compounds in the green alga Scenedesmus quadricauda. BMC Plant Biol. (2009) 9:58. 10.1186/1471-2229-9-5819445666
Adadi P Barakova NV Muravyov KY Krivoshapkina EF. Designing selenium functional foods and beverages: a review. Food Res Int. (2019) 120:708–25. 10.1016/j.foodres.2018.11.02931000289
Chan Q Afton SE Caruso JA. Selenium speciation profiles in selenite-enriched soybean (Glycine Max) by HPLC-ICPMS and ESI-ITMS. Metallomics. (2010) 2:147–53. 10.1039/B916194E21069146
Zhang X He H Xiang J Yin H Hou T. Selenium-containing proteins/peptides from plants: a review on the structures and functions. J Agric Food Chem. (2020) 68:15061–73. 10.1021/acs.jafc.0c0559433315396
Fukushima D. Recent progress of soybean protein foods: chemistry, technology, and nutrition. Food Rev Int. (1991) 7:323–51. 10.1080/87559129109540915
Ju Q Wu C Yuan Y Hu Y Zhou S Luan G. Insights into the mechanism on Glucono-delta-lactone induced gelation of soybean protein at subunit level. Food Hydrocolloids. (2022) 125:107402. 10.1016/j.foodhyd.2021.107402
Nishinari K Fang Y Guo S Phillips G. Soy proteins: a review on composition, aggregation and emulsification. Food Hydrocoll. (2014) 39:301–18. 10.1016/j.foodhyd.2014.01.013
Hu M Du X Liu G Zhang S Wu H Li Y. Germination improves the functional properties of soybean and enhances soymilk quality. Int J Food Sci Technol. (2021). 10.1111/ijfs.15461
Gao C Wang F Yuan L Liu J Sun D Li X. Physicochemical property, antioxidant activity, and cytoprotective effect of the germinated soybean proteins. Food Sci Nutr. (2019) 7:120–31. 10.1002/fsn3.82230680165
Yang H Li X Gao J Tong P Yang A Chen H. Germination-assisted enzymatic hydrolysis can improve the quality of soybean protein. J Food Sci. (2017) 82:1814–9. 10.1111/1750-3841.1378228631814
Fang G Lv Q Liu C Huo M Wang S. An ionic liquid improved HPLC-ICP-MS method for simultaneous determination of arsenic and selenium species in animal/plant-derived foodstuffs. Analyt Methods. (2015) 7:8617–25. 10.1039/C5AY02021B
Zhao X Zhao Q Chen H Xiong H. Distribution and effects of natural selenium in soybean proteins and its protective role in soybean beta-conglycinin (7S globulins) under AAPH-induced oxidative stress. Food Chem. (2019) 272:201–9. 10.1016/j.foodchem.2018.08.03930309533
Di Y Li X Chang X Gu R Duan X Liu F et al. Impact of germination on structural, functional properties and in vitro protein digestibility of sesame (Sesamum indicum L.) protein. LWT. (2022) 154:112651. 10.1016/j.lwt.2021.112651
Xu M Jin Z Peckrul A Chen B. Pulse seed germination improves antioxidative activity of phenolic compounds in stripped soybean oil-in-water emulsions. Food Chem. (2018) 250:140–7. 10.1016/j.foodchem.2018.01.04929412904
Du M Zhao L Li C Zhao G Hu X. Purification and characterization of a novel fungi Se-containing protein from Se-enriched Ganoderma Lucidum mushroom and its Se-dependent radical scavenging activity. Eur Food Res Technol. (2007) 224:659–65. 10.1007/s00217-006-0355-4
Zhang X He H Hou T. Molecular mechanisms of selenium-biofortified soybean protein and polyphenol conjugates in protecting mouse skin damaged by UV-B. Food Funct. (2020) 11:3563–73. 10.1039/C9FO02560J32270801
Luo L Zhang J Zhang K Wen Q Ming K Xiong H et al. Peanut selenium distribution, concentration, speciation, and effects on proteins after exogenous selenium biofortification. Food Chem. (2021) 354:129515. 10.1016/j.foodchem.2021.12951533756318
Zhang K Guo X Zhao Q Han Y Zhan T Li Y et al. Development and application of a HPLC-ICP-MS method to determine selenium speciation in muscle of pigs treated with different selenium supplements. Food Chem. (2020) 302:125371. 10.1016/j.foodchem.2019.12537131437711
Zhang H-J Zhang H Wang L Guo X-N. Preparation and functional properties of rice bran proteins from heat-stabilized defatted rice bran. Food Res Int. (2012) 47:359–63. 10.1016/j.foodres.2011.08.014
Zhang Q Yang L Hu S Liu X Duan X. Consequences of ball-milling treatment on the physicochemical, rheological and emulsifying properties of egg phosvitin. Food Hydrocoll. (2019) 95:418–25. 10.1016/j.foodhyd.2019.04.064
Alam MN Bristi NJ Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceut J. (2013) 21:143–52. 10.1016/j.jsps.2012.05.00224936134
Zeng R Farooq MU Zhang G Tang Z Zheng T Su Y et al. Dissecting the potential of selenoproteins extracted from selenium-enriched rice on physiological, biochemical and anti-ageing effects in vivo. Biol Trace Elem Res. (2020) 196:119–30. 10.1007/s12011-019-01896-z31786753
Deng B Tian S Li S Guo M Liu H Li Y et al. A simple, rapid and efficient method for essential element supplementation based on seed germination. Food Chem. (2020) 325:126827. 10.1016/j.foodchem.2020.12682732387939
Pyrzynska K Sentkowska A. Selenium in plant foods: speciation analysis, bioavailability, and factors affecting composition. Crit Rev Food Sci Nutr. (2021) 61:1340–52. 10.1080/10408398.2020.175802732363893
Wang Z Xie S Peng A. Distribution of Se in soybean samples with different Se concentration. J Agric Food Chem. (1996) 44:2754–9. 10.1021/jf950133h
White PJ. Selenium metabolism in plants. Biochim Biophys Acta. (2018) 1862:2333–42. 10.1016/j.bbagen.2018.05.00629751098
Dong Z Lin Y Wu H Zhang M. Selenium accumulation in protein fractions of Tenebrio molitor larvae and the antioxidant and immunoregulatory activity of protein hydrolysates. Food Chem. (2021) 334:127475. 10.1016/j.foodchem.2020.12747532688176
Liu K Chen F Zhao Y Gu Z Yang H. Selenium accumulation in protein fractions during germination of Se-enriched brown rice and molecular weights distribution of Se-containing proteins. Food Chem. (2011) 127:1526–31. 10.1016/j.foodchem.2011.02.010
Zhang B Zhou K Zhang J Chen Q Liu G Shang N et al. Accumulation and species distribution of selenium in Se-enriched bacterial cells of the Bifidobacterium animalis 01. Food Chem. (2009) 115:727–34. 10.1016/j.foodchem.2008.12.006
Wrobel K Esperanza MG Barrientos EY Escobosa ARC Wrobel K. Different approaches in metabolomic analysis of plants exposed to selenium: a comprehensive review. Acta Physiologiae Plantarum. (2020) 42:1–20. 10.1007/s11738-020-03113-0
Aijie L Shouwei Y Li L. Structure, trypsin inhibitor activity and functional properties of germinated soybean protein isolate. Int J Food Sci Technol. (2014) 49:911–9. 10.1111/ijfs.12386
Bañuelos GS Walse SS Yang SI Pickering IJ Fakra SC Marcus MA et al. Quantification, localization, and speciation of selenium in seeds of canola and two mustard species compared to seed-meals produced by hydraulic press. Anal Chem. (2012) 84:6024–30. 10.1021/ac300813e22747111
Lazo-Velez MA Mata-Ramírez D Serna-Saldivar SO Chuck-Hernandez C. Functional effects of soybean concentrates obtained from sprouted seeds enriched in selenium in wheat breadmaking. Cereal Chem J. (2017) 94:740–5. 10.1094/CCHEM-12-16-0295-R
De Wit J. Nutritional and functional characteristics of whey proteins in food products. J Dairy Sci. (1998) 81:597–608. 10.3168/jds.S0022-0302(98)75613-99565865
Kaur M Singh N. Characterization of protein isolates from different Indian chickpea (Cicer arietinum L.) cultivars. Food Chem=. (2007) 102:366–74. 10.1016/j.foodchem.2006.05.029
Shahidi F Han X-Q Synowiecki J. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chem. (1995) 53:285–93. 10.1016/0308-8146(95)93934-J
Hu Z Tang X Liu J Zhu Z Shao Y. Effect of parboiling on phytochemical content, antioxidant activity and physicochemical properties of germinated red rice. Food Chem. (2017) 214:285–92. 10.1016/j.foodchem.2016.07.09727507477
Adebowale K Lawal O. Comparative study of the functional properties of bambarra groundnut (Voandzeia subterranean), jack bean (Canavalia ensiformis) and mucuna bean (Mucuna pruriens) flours. Food Res Int. (2004) 37:355–65. 10.1016/j.foodres.2004.01.009
Vinayashree S Vasu P. Biochemical, nutritional and functional properties of protein isolate and fractions from pumpkin (Cucurbita moschata var. Kashi Harit) seeds. Food Chem. (2021) 340:128177. 10.1016/j.foodchem.2020.12817733002826
Zamani Z Razavi SM. Physicochemical, rheological and functional properties of Nettle seed (Urtica pilulifera) gum. Food Hydrocoll. (2021) 112:106304. 10.1016/j.foodhyd.2020.106304
Nir I Feldman Y Aserin A Garti N. Surface properties and emulsification behavior of denatured soy proteins. J Food Sci. (1994) 59:606–10. 10.1111/j.1365-2621.1994.tb05573.x24460091
Sun W-X Zhang R-J Fan J He Y Mao X-H. Comprehensive transformative profiling of nutritional and functional constituents during germination of soybean sprouts. J Food Measur Characterization. (2018) 12:1295–302. 10.1007/s11694-018-9743-2
Zagrodzki P Pasko P Galanty A Tyszka-Czochara M Wietecha-Posluszny R Rubio PS et al. Does selenium fortification of kale and kohlrabi sprouts change significantly their biochemical and cytotoxic properties? J Trace Elem Med Biol. (2020) 59:126466. 10.1016/j.jtemb.2020.12646631958699
Islam MZ Park BJ Kang HM Lee YT. Influence of selenium biofortification on the bioactive compounds and antioxidant activity of wheat microgreen extract. Food Chem. (2020) 309:125763. 10.1016/j.foodchem.2019.12576331787393
Guzmán-Ortiz FA San Martín-Martínez E Valverde ME Rodríguez-Aza Y Berríos JDJ Mora-Escobedo R. Profile analysis and correlation across phenolic compounds, isoflavones and antioxidant capacity during germination of soybeans (Glycine max L) CyTA. J Food. (2017) 15:516–24. 10.1080/19476337.2017.1302995
Lazo-Vélez MA Guardado-Félix D Avilés-González J Romo-López I Serna-Saldívar SO. Effect of germination with sodium selenite on the isoflavones and cellular antioxidant activity of soybean (Glycine max). LWT. (2018) 93:64–70. 10.1016/j.lwt.2018.01.060
Beermann C Euler M Herzberg J Stahl B. Anti-oxidative capacity of enzymatically released peptides from soybean protein isolate. Eur Food Res Technol. (2009) 229:637–44. 10.1007/s00217-009-1093-1
Liu K Zhao Y Chen F Fang Y. Purification and identification of Se-containing antioxidative peptides from enzymatic hydrolysates of Se-enriched brown rice protein. Food Chem. (2015) 187:424–30. 10.1016/j.foodchem.2015.04.08625977046
Zhang H Chen T Jiang J Wong YS Yang F Zheng W. Selenium-containing allophycocyanin purified from selenium-enriched Spirulina platensis attenuates AAPH-induced oxidative stress in human erythrocytes through inhibition of ROS generation. J Agric Food Chem. (2011) 59:8683–90. 10.1021/jf201976921761878
Ye Q Wu X Zhang X Wang S. Organic selenium derived from chelation of soybean peptide-selenium and its functional properties: in vitro and in vivo. Food Function. (2019) 10:4761–70. 10.1039/C9FO00729F31309961
Gobi N Vaseeharan B Rekha R Vijayakumar S Faggio C. Bioaccumulation, cytotoxicity and oxidative stress of the acute exposure selenium in Oreochromis mossambicus. Ecotoxicol Environ Saf. (2018) 162:147–59. 10.1016/j.ecoenv.2018.06.07029990726
Besse D Siedler F Diercks T Kessler H Moroder L. The redox potential of selenocystine in unconstrained cyclic peptides. Angewandte Chemie Int. (1997) 36:883–5. 10.1002/anie.199708831
Lee AL Yu YP Hsieh JF Kuo MI Ma YS Lu CP. Effect of germination on composition profiling and antioxidant activity of the polysaccharide-protein conjugate in black soybean [Glycinemax (L) Merr]. Int J Biol Macromol. (2018) 113:601–6. 10.1016/j.ijbiomac.2018.02.14529486260
