Article (Scientific journals)
Endothelial extracellular vesicles promote tumour growth by tumour-associated macrophage reprogramming.
NJOCK, Makon-Sébastien; O'Grady, Tina; Nivelles, Olivier et al.
2022In Journal of Extracellular Vesicles, 11 (6), p. 12228
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Keywords :
TAM; breast cancer; extracellular vesicles; macrophages; microRNA; MicroRNAs; Animals; Disease Models, Animal; Endothelial Cells; Mice; Tumor Microenvironment; Tumor-Associated Macrophages; Extracellular Vesicles/genetics; MicroRNAs/genetics; Neoplasms; Cell Biology; Histology
Abstract :
[en] Tumour-derived extracellular vesicles (EVs) participate in tumour progression by deregulating various physiological processes including angiogenesis and inflammation. Here we report that EVs released by endothelial cells in a mammary tumour environment participate in the recruitment of macrophages within the tumour, leading to an immunomodulatory phenotype permissive for tumour growth. Using RNA-Seq approaches, we identified several microRNAs (miRNAs) found in endothelial EVs sharing common targets involved in the regulation of the immune system. To further study the impact of these miRNAs in a mouse tumour model, we focused on three miRNAs that are conserved between humans and mouse, that is, miR-142-5p, miR-183-5p and miR-222-3p. These miRNAs are released from endothelial cells in a tumour microenvironment and are transferred via EVs to macrophages. In mouse mammary tumour models, treatment with EVs enriched in these miRNAs leads to a polarization of macrophages toward an M2-like phenotype, which in turn promotes tumour growth.
Research center :
GIGA-LAM - GIGA Cancer-Molecular Angiogenesis Laboratory - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
NJOCK, Makon-Sébastien   ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de rhumatologie
O'Grady, Tina 
Nivelles, Olivier ;  Université de Liège - ULiège > GIGA > GIGA Research (AFT)
Lion, Michelle ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique et biologie moléculaires animales
Jacques, Sophie
Cambier, Maureen  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
Herkenne, Stéphanie  ;  Université de Liège - ULiège > Département des sciences de la vie
Muller, Florian
Christian, Aurélie ;  Université de Liège - ULiège > GIGA > GIGA Cancer - Molecular Angiogenesis Laboratory
REMACLE, Claire ;  Centre Hospitalier Universitaire de Liège - CHU
GUIOT, Julien  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de pneumologie - allergologie
Rahmouni, Souad  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Dequiedt, Franck   ;  Université de Liège - ULiège > GIGA > GIGA Molecular Biology of Diseases
Struman, Ingrid   ;  Université de Liège - ULiège > Département des sciences de la vie
More authors (4 more) Less
 These authors have contributed equally to this work.
Language :
English
Title :
Endothelial extracellular vesicles promote tumour growth by tumour-associated macrophage reprogramming.
Publication date :
June 2022
Journal title :
Journal of Extracellular Vesicles
eISSN :
2001-3078
Publisher :
Wiley, United States
Volume :
11
Issue :
6
Pages :
e12228
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
ULiège - Université de Liège [BE]
Fonds Léon Fredericq [BE]
Funding number :
PDR T 1096.14
Funding text :
This study was supported by the University of Liège (ARC: RNAngio), the FNRS (Fonds National de la Recherche Scientifique, Belgium) (PDR T 1096.14), and the Léon Frédéricq fund. Computational resources have been provided by the Consortium des Équipements de Calcul Intensif (CÉCI), funded by the Fonds de la Recherche Scientifique de Belgique (F.R.S.-FNRS) under Grant No. 2.5020.11 and by the Walloon Region.
Available on ORBi :
since 17 June 2022

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