[en] Introduction
Plants of the genus Strychnos , which include about 200 species, are used for multiple traditional purposes as hunting poison, for example, and have shown interesting pharmacological properties, especially curarizing and tetanizing, but also against malaria. Many monoterpene indole alkaloids have already been isolated and identified. Among them, there is strychnine, a famous alkaloid that can cause death by asphyxiation.
Objective
Investigate alkaloidic molecular diversity from Strychnos genus using molecular networking technique and study the Strychnos genus from a chemotaxonomic point of view.
Material and methods
Twenty-eight different species and different plant parts were ground into powder using a grinder. The methanolic extracts were carried out using a pressurized solvent extraction and the alkaloid extract was performed manually with a separating funnel. The extracts were analyzed by HPLC-ESI(+)-Q/TOF. The data were processed using MZmine 2 software and the molecular network was generated on the GNPS platform. The study of the generated molecular network allowed the detection of various alkaloids. Among these is the famous strychnine which has been detected in 7 new Strychnos species not yet described as strychnine producers. This identification was investigated using orthogonal approaches, namely TLC, NMR, HPLC-UV and UHPLC-ESI(+)-Q/TOF analyses. The LOD by HPLC-UV of strychnine was also determined.
Results
Further analyses allowed to confirm the presence of strychnine in S. densiflora trunk barks but also to show the presence of strychnine with high probability in the trunk barks of S. camptoneura, S. congolana, S. boonei and S. tchibangensis and in the leaves of S. usambarensis. About the trunk barks of S. tricalyisoides , the probability of a strychnine content remains low.
Conclusion
This work exemplified the efficiency of molecular networking in identifying known metabolites (major and minor alkaloids) involved in the chemotaxonomic study of plants from Strychnos genus.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Bonnet, Olivier ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Beniddir, Mehdi A.; Université Paris-Saclay > Faculté de Pharmacie > Service de Pharmacognosie-Chimie des Substances Naturelles
Champy, Pierre; Université Paris-Saclay > Faculté de Pharmacie > Service de Pharmacognosie-Chimie des Substances Naturelles
Kagisha, Vedaste ; Université de Liège - ULiège > Faculté de Médecine > Doct. sc. bioméd. & pharma. (paysage)
Nyirimigabo, Alain; ULiège - Université de Liège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Hamann, Carla ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Jgerenaia, Giorgi ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Ledoux, Allison ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Tiabou Tchinda, Alembert; ULiège - Université de Liège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Angenot, Luc ; Université de Liège - ULiège > Département de pharmacie
Frederich, Michel ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Language :
English
Title :
Exploration by molecular networking of Strychnos alkaloids reveals the unexpected occurrence of strychnine in seven Strychnos species
Alternative titles :
[fr] L'exploration par réseau moléculaire des alcaloïdes de Strychnos révèle la présence inattendue de strychnine dans sept espèces de Strychnos
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