Subcellular Localization Of The Bovine Leukemia Virus R3 And G4 Accessory Proteins

[en] Bovine leukemia virus (BLV) is a complex retrovirus that belongs to the Deltaretrovirus genus, which also includes Human T-cell leukemia virus type 1 (HTLV-1). Both viruses contain an X region coding for at least four proteins: Tax and Rex, which are involved in transcriptional and posttranscriptional regulation, respectively, and the accessory proteins R3 and G4 (for BLV) and p12(I), p13(II), and p30(II) (for HTLV-1). The present study was aimed at characterizing the subcellular localization of BLV R3 and G4. The results of immunofluorescence experiments on transfected HeLa Tat cells demonstrated that R3 is located in the nucleus and in cellular membranes, as previously reported for HTLV-1 p12(1). In contrast, G4, like p13(II), is localized both in the nucleus and in mitochondria. In addition, we have shown that G4 harbors a mitochondrial targeting signal consisting of a hydrophobic region and an amphipathic alpha-helix. Thus, despite a lack of significant primary sequence homology, R3 and p12(1) and G4 and p13(II) exhibit similar targeting properties, suggesting possible overlap in their functional properties.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Lefebvre, Laurent

Ciminale, Vincenzo

Vanderplasschen, Alain ; Université de Liège - ULiège > Immunologie et vaccinologie

D'Agostino, Donna

Burny, Arsène ; Université de Liège - ULiège > Agro Biotech Gembloux

Willems, Luc ; Université de Liège - ULiège > GIGA-Research

Kettmann, Richard ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Language :

English

Title :

Subcellular Localization Of The Bovine Leukemia Virus R3 And G4 Accessory Proteins

Publication date :

2002

Journal title :

Journal of Virology

ISSN :

0022-538X

eISSN :

1098-5514

Publisher :

Amer Soc Microbiology, Washington, United States - Washington

Volume :

Issue :

Pages :

7843-7854

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 17 November 2010

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