[en] Silene is one of the largest genera of the world¿s flora, including around 700 species. Among them, the nocturnal moth-pollinated Silene nutans appeared to be a species complex comprising at least seven genetically differentiated lineages. In the present study floral scent and fatty acid composition of nectar of four reproductively isolated genetic lineages of S. nutans from different regions of Western Europe were evaluated to highlight differences among them. Comprehensive bidimensional gas chromatography (GC×GC) was used to allow enhanced separation of these complex samples. Floral scents were collected by trapping volatile organic compounds (VOCs) from flowering plants grown in a controlled chamber, onto sorbent-packed thermal desorption (TD) tubes placed near flowers prior separation through GC×GC and detection by mass spectrometry (MS). This analytical workflow provided a comprehensive chemical fingerprint in a single analytical run. The differences among lineages were revealed by a novel data mining and chemometrics software that enabled the extraction of useful information through powerful multivariate statistical analysis. Fatty acid (FA) profile of nectar, a very poorly investigated topic, was also evaluated. FAs, from crude nectar collected with microcapillary tubes from flowers grown under controlled conditions, were extracted and derivatised into methyl esters (FAMEs) before analysis by GC×GC and Flame Ionisation Detector. Statistical analysis was performed on FAMEs profiles to underline differences between lineages.
Eggermont, Damien ; Université de Liège - ULiège > TERRA Research Centre
Křešťáková, Veronika; Masaryk University (Czech Republic) > Faculty of Science > Department of Biochemistry
Mascrez, Steven ; Université de Liège - ULiège > Département GxABT > Chimie des agro-biosystèmes ; Université de Liège - ULiège > TERRA Research Centre
Spadafora, Natasha; University of Ferrara (Italy) > Pharmaceutical and Agricultural Sciences > Department of Chemical
Van Rossum, Fabienne; Meise botanic garden
Purcaro, Giorgia ; Université de Liège - ULiège > TERRA Research Centre > Chimie des agro-biosystèmes
Language :
English
Title :
Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques
Publication date :
17 May 2022
Event name :
Silene 2022
Event organizer :
Meise Botanic Garden Uppsala Universitet University of Gothenburg
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.