Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes
Epstein-Barr virus; Nasopharyngeal neoplasms; In situ hybridization; Polymerase chain reaction
Abstract :
[en] A case of nasopharyngeal carcinoma is presented. Epstein-Barr viral genome was identified in the neoplastic cells by in situ hybridization with digoxigenin-labelled polymerase chain reaction-generated probes. We report the development of this technique in paraffin-embedded sections and propose that such identification may prove valuable for the diagnosis of this tumour in routine material.
Disciplines :
Oncology
Author, co-author :
DELVENNE, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > > Service d'anatomie et cytologie pathologiques
KASCHTEN, Bruno ; Centre Hospitalier Universitaire de Liège - CHU > > Service de neurochirurgie
Deneufbourg, Jean-Marie ; Centre Hospitalier Universitaire de Liège - CHU > Service de radiothérapie - oncologie
Demanez, Jean-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > Service d'oto-rhino-laryngologie
Stevenaert, Achille ; Centre Hospitalier Universitaire de Liège - CHU > Service de neurochirurgie
Reznik, Michel ; Centre Hospitalier Universitaire de Liège - CHU > Service de neuropathologie
BONIVER, Jacques ; Centre Hospitalier Universitaire de Liège - CHU > > Service d'anatomie et cytologie pathologiques
Language :
English
Title :
Detection of Epstein-Barr virus in a case of undifferentiated nasopharyngeal carcinoma by in situ hybridization with digoxigenin-labelled PCR-generated probes
Publication date :
1993
Journal title :
Virchows Archiv. A: Pathological Anatomy and Histopathology
Akao I., Sato Y., Mukai K., Uhara H., Furuya S., Hoshikawa T., Shimosato Y., Takeyama I. (1991) Detection of Epstein-Barr virus DNA in formalin-fixed paraffin-embedded tissue of nasopharyngeal carcinoma using polymerase chain reaction and in situ hybridization. Laryngoscope 101:279-283.
Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C., Tuffnell P.S., Barrell B.G. (1984) DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310:207-211.
Brousset P., Butet V., Chittal S., Selves J., Delsol G. (1992) Comparison of in situ hybridization using different nonisotopic probes for detection of Epstein-Barr virus in nasopharyngeal carcinoma and immunohistochemical correlation with antilatent membrane protein antibody. Lab Invest 67:457-464.
Cai W.M., Li Y.W., Wu B., Liu Y.Y., Hu Y.H., Gu X.Z. (1983) Serologic diagnosis of nasopharyngeal carcinoma: a double-blind study of four EB virus antibodies with evaluation by sequential discrimination. Int J Radiat Oncol Biol Phys 9:1763-1768.
Chan M.K., Huang D.W., Ho Y.H., Lee J.C. (1989) Detection of Epstein-Barr virus-associated antigen in fine needle aspiration smears from cervical lymph nodes in the diagnosis of nasopharyngeal carcinoma. Acta Cytol 33:351-354.
Cheung A., Kieff E. (1982) The long internal direct repeat in Epstein-Barr virus DNA. J Virol 44:286-294.
Chu A.M., Flynn M.B., Achino E. (1984) Irradiation of nasopharyngeal carcinoma: correlation with treatment factors and stage. Int J Radiat Oncol Biol Phys 10:2241-2249.
Coates P.J., d'Ardenne A.J., Kahn G., Kangro H.O., Slavin G. (1991) Simplified procedures for applying the polymerase chain reaction to routinely fixed paraffin wax sections. J Clin Pathol 44:115-118.
Coates P.J., Mak W.P., Slavin G., d'Ardenne A.J. (1991) Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridization. J Clin Pathol 44:487-491.
Delvenne P., Engellenner W.J., Fu, Mann W.J., Chalas E., Nuovo G.J. (1992) Detection of human papillomavirus DNA in biopsyproven cervical squamous intraepithelial lesions in pregnant women. J Reprod Med 37:829-833.
Desgranges C., Wolf H., De-Thé G., Shanmugaratnam K., Cammoun N., Ellouz R., Klein K., Lennert N., Munoz N., Hausen H. (1975) Nasopharyngeal carcinoma. Presence of Epstein-Barr genomes in separated epithelial cells of tumors in patients from Singapore, Tunisia and Kenya. Int J Cancer 16:7-15.
Dickson R.I., Flores A.D. (1985) Nasopharyngeal carcinoma: an evaluation of 134 patients treated between 1971–1980. Laryngoscope 95:276-283.
Erlich H.A., Gelfand D., Sninsky J.J. (1991) Recent advances in the polymerase chain reaction. Science 252:1643-1651.
Fedder M., Gonzalez M.F. (1985) Nasopharyngeal carcinoma. Am J Med 79:365-369.
Feinberg A.P., Vogelstein B. (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6-13.
Feinmesser R., Miyazaki I., Cheung R., Freeman J.L., Noyek A.M., Dosch H.M. (1992) Diagnosis of nasopharyngeal carcinoma by DNA amplification of tissue obtained by fine-needle aspiration. N Engl J Med 326:17-21.
Feinmesser R., Feinmesser M., Freeman J.L., Noyek A.M., Livni N. (1992) Detection of occult nasopharyngeal primary tumours by means of in situ hybridization. J Laryngol Otol 106:345-348.
Furuta Y., Shinohara T., Sano K., Meguro M., Nagashima K. (1990) In situ hybridization with digoxigenin-labelled DNA probes for detection of viral genomes. J Clin Pathol 43:806-809.
Hausen H., Schulte-Holthausen H., Klein G., Henle W., Henle G., Clifford P., Santesson L. (1970) EBV DNA in biopsies of Burkitt's tumors and anaplastic carcinomas of the nasopharynx. Nature 228:1056-1058.
Hawkins E.P., Krischer J.P., Smith B.E., Hawkins H.K., Finegold M.J. (1990) Nasopharyngeal carcinoma in children — a retrospective review and demonstration of Epstein-Barr viral genomes in tumor cell cytoplasm: a report of the pediatric oncology group. Hum Pathol 21:805-810.
Henle G., Henle W. (1976) Epstein-Barr virus-specific IgA serum antibodies as an outstanding feature of nasopharyngeal carcinoma. Int J Cancer 17:1-7.
Henle W., Henle G., Ho H.C., Burtin P., Cachin Y., Clifford P., Schyryver A., de-Thé G., Diehl V., Klein G. (1970) Antibodies to Epstein-Barr virus in nasopharyngeal carcinoma, other head and neck neoplasms and control groups. J Natl Cancer Inst 44:225-231.
Huang D.P., Ho J.H.C., Henle W., Henle G. (1974) Demonstration of Epstein-Barr virus associated nuclear antigen in nasopharyngeal carcinoma cells from fresh biopsies. Int J Cancer 14:580-588.
Huth A., Estivill X., Grade K., Billwitz H., Speer A., Rosenthal A., Williaon R., Raay M., Coutelle C. (1989) Polymerase chain reaction for detection of the MP6d-9/MspI RFLP, a marker closely linked to the cystic fibrosis mutation. Nucleic Acids Res 12:7118.
Lo Y., Mehal W.Z., Fleming K.A. (1988) Rapid production of vectorfree biotinylated probes using the polymerase chain reaction. Nucleic Acids Res 16:8719.
MacComb W.S. (1972) Diagnosis and treatment of metastatic cervical cancerous nodes from an unknown primary site. Am J Surg 124:441-449.
Morris R.G., Arends M.J., Bishop P.E., Sizer K., Duvall E., Bird C.C. (1990) Sensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridization. J Clin Pathol 43:800-805.
Nonoyama M., Huang C.H., Pagano J.S., Klein G., Singh S. (1973) DNA of Epstein-Barr virus detected in tissue of Burkitt's lymphoma and nasopharyngeal carcinoma. Proc Natl Acad Sci USA 70:3265-3268.
Nuovo G.J., Richart R.M. (1989) A comparison of different in situ hybridization methodologies (biotin based and 35S based) for the detection of human papillomavirus DNA. Lab Invest 161:471-476.
Nuovo G.J., Delvenne P., McConnell P., Chalas E., Neto C., Mann W.J. (1991) Correlation of histology and detection of human papillomavirus DNA in vulvar cancers. Gynecol Oncol 43:275-280.
Old L.J., Boyse E.A., Oettgen H.F. (1966) Precipitating antibody in human serum to an antigen present in cultured Burkitt's lymphoma cells. Proceedings of the National Academy of Sciences 56:1699-1704.
Pearson G.B., Weiland L., Neel H.B., Taylor W., Earle J., Mulroney S.E., Goepfert H., Lanier A., Talvot M.L., Pilch B., Goodman M., Huang A., Levine P.H., Hya V., Moran E., Henle G., Henle W. (1983) Application of Epstein-Barr virus (EBV) serology to the diagnosis of North American nasopharyngeal carcinomas. Cancer 51:260-268.
Peiper S.C., Myers J.L., Broussard E.E., Sixbey J.W. (1990) Detection of Epstein-Barr virus genomes in archival tissues by polymerase chain reaction. Arch Pathol Lab Med 114:711-714.
Regaud C., Reverchon L. (1921) Sur un cas d'épithélioma épidermoide développé dans le massif maxillaire supérieur. Rev Laryngol Otol Rhinol 42:369-378.
Saito I., Servenius B., Compton T., Fox R.I. (1989) Detection of Epstein-Barr virus DNA by polymerase chain reaction in blood and tissue biopsies from patients with Sjögren's syndrome. J Exp Med 169:2191-2198.
Samoszuk M. (1991) Rapid detection of Epstein-Barr viral DNA by nonisotopic in situ hybridization. Correlation with the polymerase chain reaction. Am J Clin Pathol 96:448-453.
Schminke A. (1921) Über lymphoepitheliale Geschwulste. Beitr Pathol Anat Allg Pathol 68:161-170.
Shanmugaratnam K., Sobin L.H. (1978) Histological typing of upper respiratory tract tumors. International histological classification of tumors, no. 14, World Health Organization, Geneva; .
Wu T.C., Mann R.B., Epstein J.I., MacMahon E., Lee W.A., Charache P., Hayward S.D., Kurman R.J., Hayward G.S., Ambinder R.F. (1991) Abundant expression of EBER1 small nuclear RNA in nasopharyngeal carcinoma. A morphologically distinctive target for detection of Epstein-Barr virus in formalin-fixed paraffin-embedded carcinoma specimens. Am J Pathol 138:1461-1469.
Yun Z.B., Hornsleth A. (1991) Production of digoxigenin-labelled parvovirus DNA probe by PCR. Res Virol 142:277-281.
