Contribution of Mutant Analysis to the Understanding of Enzyme Catalysis: The Case of Class a Beta-Lactamases

[en] Class A beta-lactamases represent a family of well studied enzymes. They are responsible for many antibiotic resistance phenomena and thus for numerous failures in clinical chemotherapy. Despite the facts that five structures are known at high resolution and that detailed analyses of enzymes modified by site-directed mutagenesis have been performed, their exact catalytic mechanism remains controversial. This review attempts to summarize and to discuss the many available data.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Matagne, André ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie, Centre d'Ingénierie des protéines

Frère, Jean-Marie ; Université de Liège - ULiège > Département des sciences de la vie > Enzymologie, Centre d'Ingénierie des protéines

Language :

English

Title :

Contribution of Mutant Analysis to the Understanding of Enzyme Catalysis: The Case of Class a Beta-Lactamases

Publication date :

19 January 1995

Journal title :

Biochimica et Biophysica Acta

ISSN :

0006-3002

eISSN :

1878-2434

Publisher :

Elsevier, Amsterdam, Netherlands

Volume :

1246

Issue :

Pages :

109-27

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 November 2009

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137 (3 by ULiège)

Number of downloads

248 (1 by ULiège)

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Scopus citations^®

106

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

103

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