Chicory; Gut microbiota; Hormone assay; In vitro apoptosis; In vitro pro-inflammatory cytokines; Transcriptomics; Food Science; Nutrition and Dietetics
Abstract :
[en] Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects. Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete. In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones. We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses. We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation. A significant prebiotic activity was also identified. Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23–24%.
Disciplines :
Food science
Author, co-author :
Pouille, Céline L.; UMR Transfrontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—Institut Charles Viollette, Villeneuve d’Ascq, France ; Joint Laboratory CHIC41H, University of Lille-Florimond-Desprez, Villeneuve d’Ascq, France
Ouaza, Souad; UMR Transfrontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—Institut Charles Viollette, Villeneuve d’Ascq, France ; Joint Laboratory CHIC41H, University of Lille-Florimond-Desprez, Villeneuve d’Ascq, France
Roels, Elise ; UMR Transfrontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—Institut Charles Viollette, Villeneuve d’Ascq, France ; Joint Laboratory CHIC41H, University of Lille-Florimond-Desprez, Villeneuve d’Ascq, France
Behra, Josette; UMR Transfrontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—Institut Charles Viollette, Villeneuve d’Ascq, France
Tourret, Melissa; UMR Transfrontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—Institut Charles Viollette, Villeneuve d’Ascq, France
Molinié, Roland ; UMR Transfontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—BIOlogie des Plantes et Innovation (BIOPI), Amiens, France
Fontaine, Jean-Xavier ; UMR Transfontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—BIOlogie des Plantes et Innovation (BIOPI), Amiens, France
Mathiron, David ; Plateforme Analytique UFR des Sciences, UPJV, Bâtiment Serres-Transfert Rue Dallery-Passage du Sourire d’Avril, Amiens, France
Gagneul, David ; UMR Transfrontalière BioEcoAgro N◦1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV, SFR Condorcet FR CNRS 3417—Institut Charles Viollette, Villeneuve d’Ascq, France ; Joint Laboratory CHIC41H, University of Lille-Florimond-Desprez, Villeneuve d’Ascq, France
Taminiau, Bernard ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Daube, Georges ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Santé publique vétérinaire
Funding: This work has been supported by the Région des Hauts-de-France via ALIBIOTECH Institutional Review Board Statement: The study was conducted according to the Directive 2010/63/ EEC for the protection of animals used for fine scientists and in accordance with Law 2012-10 (2012)B.T., J.-X.F., D.M. and R.M.; Writing—Original Draft Preparation, A.L.-D. and C.L.P.; Writing—Review and R.M.; Formal analysis, C.L.P., A.L.-D., E.R., S.O., B.T., M.T. and D.M.; Validation, S.O.; Data and Editing, B.C., C.R., J.-L.H., B.T., G.D., J.B., R.M., D.G. and D.M.; Supervision, R.R., G.D., J.-L.H., curation, B.T., J.-X.F., D.M. and R.M.; Writing—Original Draft Preparation, A.L.-D. and C.L.P.; Writ-D.G. and C.R.; Project administration, C.R.; Funding acquisition, C.R. and J.-L.H. All authors have ing—Review and Editing, B.C., C.R., J.-L.H., B.T., G.D., J.B., R.M., D.G. and D.M.; Supervision, R.R., read and agreed to the published version of the manuscript. G.D., J.-L.H., D.G. and C.R.; Project administration, C.R.; Funding acquisition, C.R. and J.-L.H. All Funding: This work has been supported by the Région des Hauts-de-France via ALIBIOTECHThis work has been supported by the R?gion des Hauts-de-France via ALIBIOTECH CPER/FEDER 2016?2021 and FEDER for CHIC41H 2019?2021 programs.
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