[en] Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of antibody detection and the sensitivity of PCR. We have developed an immuno-quantitative PCR (iqPCR), exploiting real-time PCR technology, in order to improve this immuno-detection method and make it quantitative. To illustrate the advantages of iqPCR, we have compared it with a conventional enzyme linked immuno sorbent assay (ELISA) technique in experiments aimed at detecting the cellular and the resistant form of prion protein in bovine brain extract. The iqPCR technique proved to be more sensitive than ELISA, so it could be a technique of choice for the diagnosis of infected animals both at an ante mortem and post-mortem stage.
Gofflot, Stéphanie ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie Humaine
Elmoualij, Benaïssa ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Danièle ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Melen-Lamalle, Laurence ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Roels, Stéphane
Quatpers, Dominique
Grassi, Jacques
Vanopdenbosch, Emmanuelle
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Language :
English
Title :
Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein
Alternative titles :
[fr] Immuno PCR quantitative pour la détection et la quantification de la protéine prion
Publication date :
August 2004
Journal title :
Journal of Immunoassay and Immunochemistry
ISSN :
1532-1819
eISSN :
1532-4230
Publisher :
Marcel Dekker Inc, New York, United States - New York
Volume :
25
Issue :
3
Pages :
241-258
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
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