Reference : Determination of Benzodiazepines by Micellar Electrokinetic Chromatography
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/2761
Determination of Benzodiazepines by Micellar Electrokinetic Chromatography
English
Bechet, I. [> > > >]
Fillet, Marianne mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Hubert, Philippe mailto [Université de Liège - ULiège > > Chimie analytique >]
Crommen, Jacques mailto [Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments >]
Oct-1994
Electrophoresis
15
10
1316-21
Yes (verified by ORBi)
International
0173-0835
[en] A method for the separation and determination of benzodiazepines by micellar electrokinetic chromatography (MEKC) has been developed. Separation buffers consisted of aqueous solutions of glycine and triethanolamine (pH 9.0), containing sodium dodecyl sulfate (SDS) as surfactant and methanol as organic modifier. The effect of the concentration of SDS, methanol, glycine and triethanolamine on migration times and resolution was studied. Ten benzodiazepines were baseline separated at a 25 mM SDS concentration and 20% v/v methanol in a 75 mM glycine-250 mM triethanolamine buffer. Under these conditions, the within-day reproducibilities were 0.3-0.5% for migration times and 1.7-1.9% for peak areas at a concentration of 10 micrograms/mL. The limits of detection and quantification for oxazepam were 0.2 and 0.7 micrograms/mL, respectively, using an injection time of 5 s.
http://hdl.handle.net/2268/2761
also: http://hdl.handle.net/2268/18429
10.1002/elps.11501501200

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