Immunoglobulin Y; Salmonella spp.; in vitro adhesion; Caco-2 cells; in vitro growth
Abstract :
[en] The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. The adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial Caco-2 cell line. First, the conditions of Salmonella adherence to Caco-2 cells were optimized, and interferences of bacteria with the transepithelial electrical resistance (TER) of fully differentiated Caco-2 cell monolayers and the lactate dehydrogenase release upon exposure of the cells to Salmonella were evaluated. Both Salmonella sp. serovars were able to adhere to Caco-2 cells and decreased TER. Results from the adhesion-prevention assay demonstrated that specific IgY reduced the decrease in TER of the infected Caco-2 cell monolayers and blocked the Salmonella sp. adhesion in a concentration-dependent manner (p < 0.05). Nonspecific IgY also exhibited an inhibitory effect on these two parameters, but to a lesser extent than that of the specific IgY (p < 0.05). The protective effect of nonspecific IgY could be attributed to the low-density lipoprotein component of the water-soluble fraction of egg yolks that may not have been eliminated during ultrafiltration. The growth-inhibition assay revealed that specific IgY had an inhibitory effect on the bacterial growth, markedly during the late exponential phase, whereas nonspecific IgY failed to do so. Taken together, these results suggest that the in vitro growth inhibitory effect of specific IgY on Salmonella spp. resulted from the specific binding activity of these IgY to Salmonella sp. OMP. Passive immunization with Salmonella sp. OMP-specific IgY could thus be useful to prevent Salmonella colonization in broiler chickens and the subsequent carcass contamination during processing.
Disciplines :
Microbiology Veterinary medicine & animal health Animal production & animal husbandry
Author, co-author :
Chalghoumi, Raja
Thewis, André ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Marcq, Christopher ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Portetelle, Daniel ; Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Schneider, Yves-Jacques; Université Catholique de Louvain - UCL > Institut des Sciences de la Vie > Biochimie cellulaire, nutritionnelle et toxicologique
Language :
English
Title :
Adhesion and growth inhibitory effect of chicken egg yolk antibody (IgY) on Salmonella enterica serovars Enteritidis and Typhimurium in vitro.
Publication date :
2009
Journal title :
Foodborne Pathogens and Disease
ISSN :
1535-3141
eISSN :
1556-7125
Publisher :
Mary Ann Liebert, United States
Volume :
6
Issue :
5
Pages :
593-604
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Développement et utilisation des anticorps du jaune d'oeuf en vue du contrôle des Salmonella spp. dans le tube digestif du poulet de chair
Funders :
FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture [BE] DGA - Région wallonne. Direction générale de l'Agriculture [BE]
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