[en] Fish models are increasingly being used for hearing research investigations. Aminoglycoside antibiotics that are used for damaging the inner ear hair cells can have systemic side effects leading to death of study animals. This study aimed to compare two methods: (i) systemic (intravenous) and (ii) local (intrasaccular) gentamicin administration for induction of inner ear hair cell damage in the Atlantic cod, Gadus morhua (L.). Hair cell damage was assessed using scanning electron microscopy; hair cell density, prevalence of immature hair cells and kinocilia length were measured. Gentamicin-treated fish were compared with control and sham fish. Intravenous gentamicin led to dose-dependent mortality caused by nephrotoxicity. The only visible effect after treatment was more immature hair cells and shorter kinocilia, the effect on hair cell density was equivocal. Following intrasaccular gentamicin treatment, fish mortality was negligible, and hair cells were damaged regardless of dose. Here, we observed decreased hair cell density, high prevalence of immature hair cells, and significantly shortened kinocilia. Conclusion: intrasaccular injection is preferable to intravenous injection of gentamicin for the study of ototoxicity in the Atlantic cod.
Disciplines :
Sciences aquatiques & océanologie
Auteur, co-auteur :
Faucher, Karine ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Morphologie fonctionnelle et évolutive
Aas-Hansen, Øyvind
Damsgard, B.
Stenklev, N. C.
Langue du document :
Anglais
Titre :
Effects of systemic versus local gentamicin on the inner ear in the Atlantic cod, Gadus morhua (L.), relevance for fish hearing investigations.
Avallone B., Fascio U., Balsamo G., and Marmo F. Gentamicin ototoxicity in the saccule of the lizard Podarcis Sicula induces hair cell recovery and regeneration. Hear. Res. 235 (2008) 15-22
Chapman C.J., and Hawkins A.D. A field study of hearing in the cod, Gadus morhua L. J. Comp. Physiol. A V85 (1973) 147-167
Dale T. The labyrinthine mechanoreceptor organs of the cod Gadus morhua L. (Teleostei: Gadidae). A scanning electron microscopical study, with special reference to the morphological polarization of the macular sensory cells. Norw. J. Zool. 24 (1976) 85-128
Dulon D., Aurousseau C., Erre J.P., and Aran J.M. Relationship between the nephrotoxicity and ototoxicity induced by gentamicin in the guinea pig. Acta Otolaryngol. (Stockh.) 106 (1988) 219-225
Enger P.S., and Andersen R. An electrophysiological field study of hearing in fish. Comp. Biochem. Physiol. 22 (1967) 517-525
Faucher K., Fichet D., Miramand P., and Lagardère J.P. Impact of acute cadmium exposure on the trunk lateral line neuromasts and consequences on the "C-start" response behaviour of the sea bass (Dicentrarchus labrax L.; Teleostei, Moronidae). Aquat. Toxicol. 76 (2006) 278-294
Forge A., and Schacht J. Aminoglycoside antibiotics. Audiol. Neuro Otol. 5 (2000) 3-22
Gailiunas P., Dominguez-Moreno M., Lazrus J.M., Lowrie E.G., Gottlieb M.N., and Merril J.P. Vestibular toxicity of gentamicin: incidence in patients receiving long-term hemodialysis therapy. Arch. Intern. Med. 138 (1978) 1621-1624
Hawkins A.D. The role played by the swimbladder in the hearing in fish. J. Physiol. 227 (1972) 47P
Hiel H., Erre J.P., Aurousseau C., Bouali R., Dulon D., and Aran J.M. Gentamicin uptake by cochlear hair cells precedes hearing impairment during chronic treatment. Audiology 32 (1993) 78-87
Hudspeth A.J. Mechanoelectrical transduction by hair cells in the acousticolateralis sensory system. Ann. Rev. Neurosci. 6 (1983) 187-215
Jackson G.C., and Ancieri G. Ototoxicity of gentamicin in man: a survey and controlled analysis of clinical experience in the U.S. J. Infect. Dis. Suppl. 124 (1971) 130-137
Kaus S. The effect of aminoglycoside antibiotics on the lateral line organ of Aplocheilus lineatus (Cyprinodontidae). Acta Otolaryngol. (Stockh.) 103 (1987) 291-298
Kroese A.B.A., Das A., and Hudspeth A.J. Blockage of the transduction channels of hair cells in the bullfrog's sacculus by aminoglycoside antibiotics. Hear. Res. 37 (1989) 203-217
Liu M., Reimschuessel R., and Hassel B.A. Molecular cloning of the fish interferon stimulated gene, 15 kDa (ISG15) orthologue: a ubiquitin-like gene induced by nephrotoxic damage. Gene 298 (2002) 129-139
Lombarte A., Yan H.Y., Popper A.N., Chang J.S., and Platt C. Damage and regeneration of hair cell ciliary bundles in a fish ear following treatment with gentamicin. Hear. Res. 64 (1993) 166-174
Matsuura S., Ikeda K., and Furukawa T. Effects of streptomycin, kanamycin, quinine, and other drugs on the microphonic potentials of goldfish sacculus. Jap. J. Physiol. 21 (1971) 579-590
Nakagawa T., Kim T.S., Murai N., Endo T., Iguchi F., Tateya I., Yamamoto N., Naito Y., and Ito J. A novel technique for inducing local inner ear damage. Hear. Res. 176 (2003) 122-127
Presson J.C., and Popper A.N. Possible precursors to new hair cells, support cells, and Schwann cells in the ear of a post-embryonic fish. Hear. Res. 46 (1990) 9-21
Rouse G.W., and Pickles J.O. Paired development of hair cells in neuromasts of the teleost lateral line. Proc. R. Soc. Lond. B 246 (1991) 123-128
Sand O., and Enger P.S. Evidence for an auditory function of the swimbladder in the cod. J. Exp. Biol. 59 (1973) 405-414
Scholik A.R., and Yan H.Y. Effects of underwater noise on auditory sensitivity of a cyprinid fish. Hear. Res. 152 (2001) 17-24
Schönleber J., and Anken R.H. Efficacy of an ototoxic aminoglycoside (gentamicin) on the differentiation of the inner ear of cichlid fish. Adv. Space Res. 33 (2004) 1416-1420
Schuijf A. Directional hearing of cod (Gadus morhua) under approximate free field conditions. J. Comp. Physiol. A. V98 (1975) 307-332
Schuijf A., and Buwalda R.J.A. On the mechanism of directional hearing in cod (Gadus morhuaL.). J. Comp. Physiol. A. V98 (1975) 333-343
Schuijf A., and Hawkins A.D. Acoustic distance discrimination by the cod. Nature 302 (1983) 143-144
Smith M.E., Coffin A.B., Miller D.L., and Popper A.N. Anatomical and functional recovery of the goldfish (Carassius auratus) ear following noise exposure. J. Exp. Biol. 209 (2006) 4193-4202
Song J., Young Yan H., and Popper A.N. Damage and recovery of hair cells in fish canal (but not superficial) neuromasts after gentamicin exposure. Hear. Res. 91 (1995) 63-71
Wersäll J., and Flock A. Suppression and restoration of the microphonic output from the lateral line organ after local application of streptomycin. Life Sci. 3 (1964) 1151-1155
Yan H.Y., Saidel W.M., Chang J.S., Presson J.C., and Popper A.N. Sensory hair cells of a fish ear: evidence of multiple types based on ototoxicity sensitivity. Proc. R. Soc. Lond. B 245 (1991) 133-138
