[en] The phenomenon of ozone tolerance is described, but the underlying mechanisms remain unknown. We tested whether adaptation to multiday ozone exposure was related to an upregulated pulmonary antioxidant defence. Six calves were exposed to 0.75 ppm ozone, 12 h day(-1) for seven consecutive days. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed before, after the first (D1), third (D3) and seventh (D7) exposure. Differential cell count, total proteins, 8-epi-PGF2alpha, glutathione and uric acid were determined in BAL. Dynamic lung compliance and arterial oxygen tension were significantly decreased and lung oedema impaired pulmonary function on D1. By repeating ozone exposures, progressive functional adaptation occurred. Ozone induced a significant increase of BAL neutrophil percentage on D1. On D3 and D7, neutrophil percentage was progressively decreased, but remained significantly elevated. BAL total proteins were significantly increased on D1 and decreased progressively until D7. 8-Epi-PGF2alpha was significantly increased on D1 and was returned to baseline on D3 and D7, whilst glutathione significantly increased on D3 and returned to baseline on D7. Uric acid was increased ten-fold on D1. On D3, uric acid was increased six-fold and was persistently elevated at D7. This study suggests that ozone adaptation of functional and inflammatory variables is accompanied with sustained BAL uric acid elevation.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology Veterinary medicine & animal health
Author, co-author :
Kirschvink, Nathalie; Université de Liège - ULiège
Fievez, Laurence ; Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Bureau, Fabrice ; Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Degand, Guy ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Maghuin-Rogister, Guy ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Département de sciences des denrées alimentaires
Smith, Nicola; Centre for Equine Studies, Animal health trust, Newmarket, UK
Art, Tatiana ; Université de Liège - ULiège > Département de sciences fonctionnelles > Phys. neuro-muscul., de l'effort - Méd. sport. des animaux
Lekeux, Pierre ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie - Doyen de la Faculté de Médecine vétérinaire
Language :
English
Title :
Adaptation to multiday ozone exposure is associated with a sustained increase of bronchoalveolar uric acid.
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Bibliography
Cross C.E., van der Vliet J.P., Eiserich J., Wong J., Halliwell B. (1997) "Oxidative stress and antioxidants in respiratory tract lining fluids". Lung Biology in Health and Disease Oxygen, Gene Expression, and Cellular Function , Clerch, L.B. and Massaro, D.J., eds, (Marcel Dekker, New York); 105:367-398.
(1996) "Health effects of outdoor air pollution". Am. J. Respir. Critical Care Med , Committee of the Environmental and Occupational Health Assembly of the American Thoracic Society Bascom, R., et al; 153:3-50.
Aris R.M., Christian D., Hearne P.Q., Kerr K., Pinkbeiner W.E., Balmes J.R. (1993) "Ozone-induced airway inflammation in human subjects as determined by airway lavage and biopsy". Am. Rev. Respir. Dis 148:1363-1372.
Weinmann G.G., Liu M.C., Proud D., Weidenbach-Gerbase M., Hubbard W., Frank R. (1995) "Ozone exposure in humans: Inflammatory, small and peripheral airway responses". Am. J. Respir. Critical Care Med 152:1175-1182.
Balmes J.R., Chen L.L., Scannell C., Tager I., Christian D., Hearne P.Q., Kelly T.H., Aris R.M. (1996) "Ozone-induced decrements in FEV1 and FVC do not correlate with measures of inflammation". Am. J. Respir. Critical Care Med 153:904-909.
Blomberg A., Mudway I.S., Nordenhäll C., Hedenström H., Kelly F.J., Frew A.J., Holgate S.T., Sandström T. (1999) "Ozone-induced lung function decrements do not correlate with early airway inflammation or antioxidant responses". Eur. Respir. J 13:1418-1428.
Kelly F.J., Cotgrove M., Mudway I.S. (1996) "Respiratory tract lining fluid antioxidants: The first line of defence against gaseous pollutants". Cent. Eur. J. Public Hlth 4(SUPPL.):11-14.
Kodavanti U.P., Costa D.L., Richards J., Crissman K.M., Slade R., Hatch G.E. (1996) "Antioxidants in bronchoalveolar lavage fluid cells isolated from ozone-exposed normal and ascorbate-deficient guinea pigs". Exp. Lung Res 22:435-448.
Mudway I.S., Housley D., Eccles R., Richards R.J., Datta A.K., Tetley T.D., Kelly F.J. (1996) "Differential depletion of human respiratory tract antioxidants in response to ozone challenge". Free Rad 25:499-513.
Hulsmann A.R., Raatgeep H.R., den Hollander J.C., Stijnen T., Saxena P.R., Kerrebijn K.F., de Jongste J.C. (1994) "Oxidative epithelial damage produces hyperresponsiveness of human peripheral airways". Am. J. Respir. Critical Care Med 149:519-525.
Kafoury R.M.M., Pryor W.A., Squadrito G.L., Salgo M.G., Zou X., Friedman M. (1999) "Induction of inflammatory mediators in human airway epithelial cells by lipid ozonation products". Am. J. Respir. Critical Care Med 160:1934-1942.
Morrow J.D., Roberts L.J. II (1996) "The isoprostanes. Current knowledge and directions for future research". Biochem. Pharmacol 51:1-9.
Lawson J.A., Rokach J., FitzGerald G.A. (1999) "Isoprostanes: Formation, analysis and use as indices of lipid peroxidation in vivo". J. Biol. Chem 274:24441-24444.
Montuschi P., Ciabattoni G., Paredi P., Pantelidis P., du Bois R.M., Kharitonov S.A., Barnes P.J. (1998) "8-Isoprostane as a biomarker of oxidative stress in interstitial lung diseases". Am. J. Respir. Critical Care Med 158:1524-1527.
Montuschi P., Corradi M., Ciabattoni G., Nightingale J., Kharitonov S.A., Barnes P.J. (1999) "Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients". Am. J. Respir. Critical Care Med 160:216-220.
Hazbun M.E., Hamilton R., Holian A., Eschenbacher W.L. (1993) "Ozone-induced increases in substance P and 8-epi-prostaglandin F2α in the airways of human subjects". Am. J. Respir. Cell Mol. Biol 9:568-572.
Kawikova I., Barnes P.J., Takahashi T., Tadjkarimi S., Yacoub M.H., Belvisi M.G. (1996) "8-Epi-PGF2α, a novel noncyclooxygenase-derived prostaglandin, constricts airways in vitro". Am. J. Respir. Critical Care Med 153:590-596.
Okazawa A., Kawikova I., Cui Z.-H., Skoogh B.-E., Lötvall J. (1997) "8-Epi-PGF2α induces airflow obstruction and airway plasma exudation in vivo". Am. J. Respir. Critical Care Med 155:436-441.
Avissar N.E., Reed C.K., Cox C., Frampton M.W., Finkelstein J.N. (2000) "Ozone, but not nitrogen dioxide, exposure decreases glutathione peroxidases in epithelial lining fluid of human lung". Am. J. Respir. Critical Care Med 162:1342-1347.
Jörres R.A., Holz O., Zachgo W., Timm P., Koschyk S., Müller B., Grimminger F., Seeger W., Kelly C., Dunster T., Frischer G., Lubec M., Waschewski A., Niendorf H. (2000) "The effect of repeated ozone exposures on inflammatory markers in bronchoaveolar lavage fluid and mucosal biopsies". Am. J. Respir. Critical Care Med 161:1855-1861.
Coghe J., Uystepruyst Ch., Bureau F., Detilleux J., Art T., Lekeux P. (2000) "Validation and prognostic value of plasma lactate measurement in bovine respiratory disease". Vet. J 160:139-146.
Lekeux P., Hajer R., Breukink H.J. (1984) "Intrathoracic pressure measurement in cattle: Standardized procedure". Can. J. Comp. Med 48:420-421.
Art T., Duvivier D.H., Votion D., Anciaux N., Vandenput S., Bayly W.M., Lekeux P. (1998) "Does an acute COPD crisis modify the cardiorespiratory and ventilatory adjustments to exercise in horses?". J. Appl. Physiol 84:845-852.
Grootveld M., Halliwell B. (1987) "Measurement of allantoin and uric acid in human body fluids. A potential index of free-radical reactions in vivo?". Biochem. J 243:803-808.
Smith N.C., Dunnett M., Mills P.C. (1995) "Simultaneous quantitation of oxidised and reduced glutathione in equine biological fluids by reversed-phase high-performance liquid chromatography using electrochemical detection". J. Chromatogr. B: Biomed. Appl 673:35-41.
Krombach F., Fiehl E., Burkhardt D., Rienmuller R., Konig G., Adelmann-Grill B.C., Idel H., Rosenbruch M. (1994) "Short-term and long-term effects of serial bronchoalveolar lavages in a nonhuman primate". Am. J. Respir. Critical Care Med 150:153-158.
Yokoyama E., Goto H., Kawai K., Kyono H. (1989) "Mechanical properties of rabbit lung with edema caused by exposure to ozone". J. Environ. Pathol. Toxicol. Oncol 9:95-108.
Christian D.L., Chen L.L., Scannell C.H., Ferrando R.E., Welch B.S., Balmes J.R. (1998) "Ozone-induced inflammation is attenuated with multiday exposure". Am. J. Respir. Critical Care Med 158:532-537.
Holz O., Jörres R.A., Timm P., Mücke M., Richter K., Koschyk S., Magnussen H. (1999) "Ozone-induced airway inflammatory changes differ between individuals and are reproducible". Am. J. Respir. Critical Care Med 159:776-784.
Lekeux P., Hajer R., van den Ingh T.S.G.A.M., Breukink H.J. (1985) "Pathophysiologic study of 3-methylindole-induced pulmonary toxicosis in immature cattle". Am. J.Vet. Res 46:1629-1631.
Farrell B.P., Kerr H.D., Kulle T.J., Sauder L.R., Young J.L. (1979) "Adaptation in human subjects to the effects of inhaled ozone after repeated exposure". Am. Rev. Respir. Dis 119:725-730.
Haslett C. (1999) "Granulocyte apoptosis and its role in the resolution and control of lung inflammation". Am. J. Respir. Critical Care Med 160.
Mills P.C., Roberts C.A., Smith N.C. (1996) "Effects of ozone and airway inflammation on glutathione status and iron homeostasis in the lungs of horses". Am. J. Vet. Res 57:1359-1363.
Boehme D.S., Hotchkiss J.A., Henderson R.F. (1992) "Glutathione and GSH-dependent enzymes in bronchoalveolar lavage fluid cells in response to ozone". Exp. Mol. Pathol 56:37-48.
Mudway I.S., Krishna M.T., Frew A.J., MacLeod D., Sandstrom T., Holgate S.T., Kelly F.J. (1999) "Compromised concentrations of ascorbate in fluid lining the respiratory tract in human subjects after exposure to ozone". Occup. Environ. Med 56:473-481.
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