Triterpenoid and steroid coumponds isolated from leaves of Hyperacanthus thouvenotii (Rubiaceae), endemic plant from Madagascar

Madagascar is well known for its biodiversity. Its flora is exceptional, with an endemism rate of more than 80 %. Many plants have therapeutic potential. However many of them have not been studied. Those include the many endemic species like Hyperacathus thouvenotii (Rubiaceae) which has neither been studied for its chemical composition nor its biological activities.
The aims of this study were to isolate and indentify compounds from Hyperacathus thouvenotii in order to valorize the plant and its molecules for therapeutic and chemotaxonomic purposes.
To isolate, a weighed mass of air-dried and powdered leaves of the plant were extracted by maceration successively at room temperature with dichloromethan and methanol. The fractionation and isolation were carried out using an open column packed with silica gel. The chemical structures were elucidated by using GC/MS and NMR spectroscopy.
Seven known coumponds have identified for the first time in Hyperacathus thouvenotii. Those are β-sitosterol, ursolic acid, uvaol, moretenol, 24-hydroxy α-amyrin, 24-hydroxy β amyrin in dichloromethan extract and β-sitosterol-3-O-glucoside in methalonic extract