Electronic and Molecular Properties of an Adsorbed Protein Monolayer Probed by Two-Color Sum-Frequency Generation Spectroscopy

Dreesen, Laurent; Humbert, C.; Sartenaer, Y.; Caudano, Y.; Volcke, C.; Mani, A. A.; Peremans, A.; Thiry, P. A.; Hanique, S.; Frère, Jean-Marie

doi:10.1021/la0488001

Article (Scientific journals)

Electronic and Molecular Properties of an Adsorbed Protein Monolayer Probed by Two-Color Sum-Frequency Generation Spectroscopy

Dreesen, Laurent; Humbert, C.; Sartenaer, Y. et al.

2004 • In Langmuir, 20 (17), p. 7201-7207

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https://hdl.handle.net/2268/26725

DOI
10.1021/la0488001

PubMed
15301506

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Abstract :

[en] Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.

Disciplines :

Physics

Author, co-author :

Dreesen, Laurent ; Université de Liège - ULiège > Département de physique > Biophotonique

Humbert, C.

Sartenaer, Y.

Caudano, Y.

Volcke, C.

Mani, A. A.

Peremans, A.

Thiry, P. A.

Hanique, S.

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Electronic and Molecular Properties of an Adsorbed Protein Monolayer Probed by Two-Color Sum-Frequency Generation Spectroscopy

Publication date :

2004

Journal title :

Langmuir

ISSN :

0743-7463

eISSN :

1520-5827

Publisher :

American Chemical Society, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

7201-7207

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 26 October 2009

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