Extracellular Vesicles Mediate Communication between Endothelial and Vascular Smooth Muscle Cells.

[en] The recruitment of pericytes and vascular smooth muscle cells (SMCs) that enwrap endothelial cells (ECs) is a crucial process for vascular maturation and stabilization. Communication between these two cell types is crucial during vascular development and in maintaining vessel homeostasis. Extracellular vesicles (EVs) have emerged as a new communication tool involving the exchange of microRNAs between cells. In the present study, we searched for microRNAs that could be transferred via EVs from ECs to SMCs and vice versa. Thanks to a microRNA profiling experiment, we found that two microRNAs are more exported in each cell type in coculture experiments: while miR-539 is more secreted by ECs, miR-582 is more present in EVs from SMCs. Functional assays revealed that both microRNAs can modulate both cell-type phenotypes. We further identified miR-539 and miR-582 targets, in agreement with their respective cell functions. The results obtained in vivo in the neovascularization model suggest that miR-539 and miR-582 might cooperate to trigger the process of blood vessel coverage by smooth muscle cells in a mature plexus. Taken together, these results are the first to highlight the role of miR-539 and miR-582 in angiogenesis and communication between ECs and SMCs.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Fontaine, Marie

Herkenne, Stéphanie ; Université de Liège - ULiège > GIGA Cancer - Molecular Angiogenesis Laboratory

Ek, Olivier ; Université de Liège - ULiège > Plateforme Diagnostic Covid-19

Paquot, Alicia

Boeckx, Amandine ; Université de Liège - ULiège > GIGA Cancer - Molecular Angiogenesis Laboratory

Paques, Cécile

Nivelles, Olivier

Thiry, Marc ; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Struman, Ingrid ; Université de Liège - ULiège > GIGA Cancer - Molecular Angiogenesis Laboratory

Language :

English

Title :

Extracellular Vesicles Mediate Communication between Endothelial and Vascular Smooth Muscle Cells.

Publication date :

2022

Journal title :

International Journal of Molecular Sciences

ISSN :

1661-6596

eISSN :

1422-0067

Publisher :

Multidisciplinary Digital Publishing Institute (MDPI), Switzerland

Volume :

Issue :

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 16 January 2022

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