Article (Scientific journals)
In-Depth Longitudinal Comparison of Clinical Specimens to Detect SARS-CoV-2.
Defêche, Justine; AZARZAR, Samira; Mesdagh, Alyssia et al.
2021In Pathogens (Basel, Switzerland), 10 (11)
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Keywords :
COVID-19; SARS-CoV-2; diagnosis; persistence
Abstract :
[en] The testing and isolation of patients with coronavirus disease 2019 (COVID-19) are indispensable tools to control the ongoing COVID-19 pandemic. PCR tests are considered the "gold standard" of COVID-19 testing and mostly involve testing nasopharyngeal swab specimens. Our study aimed to compare the sensitivity of tests for various sample specimens. Seventy-five participants with confirmed COVID-19 were included in the study. Nasopharyngeal swabs, oropharyngeal swabs, Oracol-collected saliva, throat washes and rectal specimens were collected along with pooled swabs. Participants were asked to complete a questionnaire to correlate specific clinical symptoms and the symptom duration with the sensitivity of detecting COVID-19 in various sample specimens. Sampling was repeated after 7 to 10 days (T2), then after 14 to 20 days (T3) to perform a longitudinal analysis of sample specimen sensitivity. At the first time point, the highest percentages of SARS-CoV-2-positive samples were observed for nasopharyngeal samples (84.3%), while 74%, 68.2%, 58.8% and 3.5% of throat washing, Oracol-collected saliva, oropharyngeal and rectal samples tested positive, respectively. The sensitivity of all sampling methods except throat wash samples decreased rapidly at later time points compared to the first collection. The throat washing method exhibited better performance than the gold standard nasopharyngeal swab at the second and third time points after the first positive test date. Nasopharyngeal swabs were the most sensitive specimens for early detection after symptom onset. Throat washing is a sensitive alternative method. It was found that SARS-CoV-2 persists longer in the throat and saliva than in the nasopharynx.
Disciplines :
Immunology & infectious disease
Author, co-author :
Defêche, Justine ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Pool assistant - biologie clinique
AZARZAR, Samira ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service des maladies infectieuses - médecine interne
Mesdagh, Alyssia ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service de rhumatologie
DELLOT, Patricia ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service des maladies infectieuses - médecine interne
Tytgat, Amandine ;  Université de Liège - ULiège > GIGA I3 - Cellular and Molecular Immunology
Bureau, Fabrice ;  Université de Liège - ULiège > Vice-Recteur à la Recherche
Gillet, Laurent  ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Vaccinologie vétérinaire
Belhadj, Yasmine
BONTEMS, Sébastien ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Laboratoire LRS
HAYETTE, Marie-Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > Unilab > Service de microbiologie clinique
Schils, Raphaël ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service des maladies infectieuses - médecine interne
Rahmouni, Souad  ;  Université de Liège - ULiège > GIGA Medical Genomics - Unit of Animal Genomics
ERNST, Marie  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de gestion des systèmes d'informations (GSI) > Secteur d'appui à la recherche clinique et biostatistique
MOUTSCHEN, Michel  ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service des maladies infectieuses - médecine interne
DARCIS, Gilles ;  Centre Hospitalier Universitaire de Liège - CHU > Département de médecine interne > Service des maladies infectieuses - médecine interne
More authors (5 more) Less
Language :
English
Title :
In-Depth Longitudinal Comparison of Clinical Specimens to Detect SARS-CoV-2.
Publication date :
2021
Journal title :
Pathogens (Basel, Switzerland)
ISSN :
2076-0817
eISSN :
2076-0817
Volume :
10
Issue :
11
Peer reviewed :
Peer reviewed
Available on ORBi :
since 14 December 2021

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