Reference : Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability ...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Development Of An Enzymatic Assay For The Determination Of Cellulose Bioavailability In Municipal Solid Waste
Rodriguez, Christian [> >]
Hiligsmann, Serge mailto [Université de Liège - ULiège > > Biochimie et microbiologie industrielles >]
Ongena, Marc mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Charlier, Robert mailto [Université de Liège - ULiège > Département Argenco : Secteur GEO3 > Géomécanique et géologie de l'ingénieur >]
Thonart, Philippe mailto [Université de Liège - ULiège > > Gembloux Agro-Bio Tech >]
Yes (verified by ORBi)
[en] biodegradation ; biochemical methane potential assay ; municipal solid waste ; cellulosic compounds ; enzymatic hydrolysis test
[en] As there is a constant need to assess the biodegradation potential of refuse disposed of
in landfills, we have developed a method to evaluate the biodegradability of cellulosic
compounds (cellulose and hemicellulose) in municipal solid waste. This test is based on
the quantification of monosaccharids released after the hydrolysis of solid waste
samples with an optimised enzyme preparation containing commercially available
cellulases and hemicellulases. We show that the amounts of monosaccharids could be
related to the biodegradability of the cellulosic material contained in the samples. This
enzymatic cellulose degradation test was assayed on 26 samples originating from two
Belgian landfills and collected at different depths. As results correlated well with those
obtained with a classical biochemical methane potential assay, this new and rapid test is
sufficiently reliable to evaluate cellulose bioavailability in waste samples.
Centre Wallon de Biologie Industrielle
Communauté française - Action de recherche concertée
Confinement de centres d'enfouissement technique à l'aide de barrières argileuses
Researchers ; Professionals ; Students ; General public

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