Article (Scientific journals)
Sixty years from the first disease description, a novel badnavirus associated with chestnut mosaic disease.
Marais, Armelle; Murolo, Sergio; Faure, Chantal et al.
2021In Phytopathology
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Keywords :
Etiology; Pathogen Detection; Virology
Abstract :
[en] Although the chestnut mosaic disease (ChMD) was described several decades ago, its etiology is still not elucidated. Here, using classical approaches in combination with high throughput sequencing (HTS) techniques, we identify a novel Badnavirus that is a strong etiological candidate for ChMD. Two disease sources from Italy and France were submitted to HTS-based viral indexing. Total RNAs were extracted, ribodepleted and sequenced on an Illumina NextSeq500 (2x150 or 2x 75 nt). In each source, we identified a single contig of about 7.2 kilobases that corresponds to a complete circular viral genome and shares homologies with various badnaviruses. The genomes of the two isolates have an average nucleotide identity of 90.5% with a typical badnaviral genome organization comprising three open reading frames. Phylogenetic analyses and sequence comparisons show that this virus is a novel species for which we propose the name Chestnut mosaic virus (ChMV). Using a newly developed molecular detection test, we systematically detected the virus in symptomatic graft-inoculated indicator plants (chestnut and American oak), as well in chestnut trees presenting typical ChMD symptoms in the field (100% and 87% in France and Italy surveys, respectively). Datamining of publicly available chestnut SRA transcriptomic data allowed the reconstruction of two additional complete ChMV genomes from two Castanea mollissima sources from the USA, as well as ChMV detection in C. dentata from the USA. Preliminary epidemiological studies, performed in France and in Central Eastern Italy, showed that ChMV has a high incidence in some commercial orchards, with a low within-orchard genetic diversity.
Disciplines :
Agriculture & agronomy
Author, co-author :
Marais, Armelle
Murolo, Sergio
Faure, Chantal
Brans, Yoann
Larue, Clement
Maclot, François  ;  Université de Liège - ULiège > Terra
Massart, Sébastien  ;  Université de Liège - ULiège > Département GxABT > Gestion durable des bio-agresseurs
Chiumenti, Michela
Minafra, Angelantonio
Romanazzi, Gianfranco
Lefebvre, Marie
Barreneche, Teresa
Robin, Cécile
Petit, Remy J.
Candresse, Thierry
More authors (5 more) Less
Language :
English
Title :
Sixty years from the first disease description, a novel badnavirus associated with chestnut mosaic disease.
Publication date :
2021
Journal title :
Phytopathology
ISSN :
0031-949X
eISSN :
1943-7684
Publisher :
American Phytopathological Society, United States
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 09 September 2021

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