Article (Scientific journals)
Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes
Mross, S.; Zimmermann, T.; Zenzes, S. et al.
2017In Sensors and Actuators. B, Chemical, 241, p. 48-54
Peer Reviewed verified by ORBi
 

Files


Full Text
1-s2.0-S092540051631680X-main.pdf
Publisher postprint (2.49 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Bioprocess monitoring; Enzyme sensors; In-situ monitoring; Polyurethane membrane; Surface morphology; Biotechnology; Enzymes; Glucose; Membranes; Polyurethanes; Yeast; Biotechnological process; Electron beam irradiation; Fabrication parameters; In- situ monitoring; Long term performance; Polymer concentrations; Polyurethane membranes
Abstract :
[en] We report on a study of enzymatic glucose and lactate sensors for measurements of wide concentration ranges from 1 mM up to 600 mM and 900 mM, respectively, in biotechnological processes. Diffusion-limiting polyurethane membranes were used to extend the linear measurement range and the influence of two fabrication parameters on membrane properties and sensor performance evaluated. The polymer concentration was varied between 4% and 10% and the ratio of the solvents tetrahydrofuran and dimethylformamide between 9:1 and 1:9. Surface morphology and permeability of the membranes and the linear measurement range of membrane-covered enzyme sensors were determined. A distinct relationship between the different results was found. The sensors retained their functionality after being sterilized using gamma and electron beam irradiation. The long-term performance of the sensors was evaluated and good performance in the glucose monitoring of a culture of Saccharomyces cerevisiae over 13 days was shown. © 2016 Elsevier B.V.
Disciplines :
Electrical & electronics engineering
Author, co-author :
Mross, S.;  Fraunhofer Institute for Microelectronic Circuits and Systems, Finkenstrasse 61, Duisburg, 47057, Germany
Zimmermann, T.;  Fraunhofer Institute for Microelectronic Circuits and Systems, Finkenstrasse 61, Duisburg, 47057, Germany
Zenzes, S.;  University of Applied Sciences Niederrhein, Krefeld, Germany
Kraft, Michael ;  Université de Liège - ULg
Vogt, H.;  Fraunhofer Institute for Microelectronic Circuits and Systems, Finkenstrasse 61, Duisburg, 47057, Germany
Language :
English
Title :
Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes
Publication date :
2017
Journal title :
Sensors and Actuators. B, Chemical
ISSN :
0925-4005
eISSN :
1873-3077
Publisher :
Elsevier Sequoia, Lausanne, Switzerland
Volume :
241
Pages :
48-54
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 September 2021

Statistics


Number of views
34 (0 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
4
Scopus citations®
without self-citations
3
OpenCitations
 
3

Bibliography


Similar publications



Contact ORBi