Pentas longiflora Oliv. (Rubiaceae), a plant used in the treatment of Pityriasis Versicolor in Rwanda: Chemical composition and standardization of leaves and roots
[en] In Rwanda, the roots of Pentas longiflora Oliv. (Rubiaceae) have been used for a long time to treat Pityriasis versicolor. However, many people reported the use of leaves instead of roots. This research was conducted to compare the phytochemical composition and establish chromatographic methods for the standardization of roots and leaves extracts of P. longiflora. During this process, three new pentalongin glycosides (pentalonginoside A, pentalonginoside B, and pentalonginoside C) and two known glycosides of the same type (harounoside and clarinoside), as well as rutin, luteolin-7-rutinoside were isolated from methanol extract of leaves. In addition, pentalongin and psychorubrin, previously isolated from ethylacetate roots extract, were also identified in Pentas longiflora ethylacetate leaves extract. The presence of the antifungal compound pentalongin in leaves may explain the traditional use of leaves in the treatment of Pytiriasis versicolor. Furthermore, harounoside, psychorubrin, and pentalongin were selected as markers for HPLC fingerprints of MeOH extract. The accuracy and risk profile demonstrated the reliability of the validated method. In general, considerable variations of concentration in plant metabolites, including pentalongin, were observed between samples from different sites. The content in pentalongin (expressed as juglone) in collected samples ranged between 1.7 and 70.0 mg/100 g. The highest concentration (70.0 ± 17 mg/100 g) was registered in the cultivated samples from Mukoni. This important variation of pentalongin concentrations according to sampling sites, shows that in order to guarantee equivalent efficacy, finished products with P. longiflora should be standardized based on their pentalongin content.
Bonnet, Olivier ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Tchinda, Alembert Tiabou
Jansen, Olivia ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Tomani, Jean Claude
Ranarivelo, Njakarinala ; Université de Liège - ULiège > Unités de recherche interfacultaires > Centre Interdisciplinaire de Recherche sur le Médicament (CIRM)
Ledoux, Allison ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Nyirimigabo, Alain
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Language :
English
Title :
Pentas longiflora Oliv. (Rubiaceae), a plant used in the treatment of Pityriasis Versicolor in Rwanda: Chemical composition and standardization of leaves and roots
Giusiano, G., de los Sosa, M.A., Rojas, F., Vanacore, S.T., Mangiaterra, M., Prevalencia de especies de Malassezia en lesiones de pitiriasis versicolor en el nordeste argentino. Rev. Iberoam. Micol. 27:2 (2010), 71–74, 10.1016/j.riam.2009.12.005.
Ravindranath, S., Pityriasis versicolor: therapeutic efficacy of various regimes of topical 2% clotrimazole cream, oral flucanazole and ketoconazole. Int. J. Contemp. Med. Res. 3:8 (2016), 2355–2360.
Kumar Rai, M., Wankhade, S., Tinea versicolor - an epidemiology. J. Microb. Biochem. Technol. 01:01 (2009), 051–056, 10.4172/1948-5948.1000010.
Snekavalli, R., Madhu, R., Ramesh, A., Janaki, C., Dhanalakshmi, U.R., Clinico epidemiological and mycological study of pityriasis versicolor PITYRIASIS VERSICOLOR. Int. J. Res. Med. Sci., 6(6), 2018, 1963, 10.18203/2320-6012.ijrms20182043.
Rojas, F.D., Sosa, M. De Los A., Fernández, M.S., Cattana, M.E., Córdoba, S.B., Giusiano, G.E., Antifungal susceptibility of Malassezia furfur, Malassezia sympodialis, and Malassezia globosa to azole drugs and amphotericin B evaluated using a broth microdilution method. Med. Mycol. 52:6 (2014), 641–646, 10.1093/mmy/myu010.
Nura, P., Sani, P., Abubakar, P., Kutama, P., A review of the current status of tinea versicolor in some parts of Nigeria. Int. J. Sci. Eng. Appl. Sci. 2:1 (2016), 2395–3470 [Online]. Available: www.ijseas.com.
Hostettman, K., Chinyanganya, F., Maillard, M., Wolfender, J.-L., Working Group on Plant Chemistry. 1996, 243–251 [Online]. Available: https://pdfs.semanticscholar.org/b48c/8f05bd1efc1dd3b3449268c44242f2f441e4.pdf.
Claessens, S., et al. The behaviour of the natural pyranonaphthoquinone pentalongin in alcoholic solvents. Tetrahedron 62:21 (2006), 5152–5158, 10.1016/j.tet.2006.03.018.
Sweelam, H.M., Abd-alla, H.I., Abdelwahab, A.B., Gabr, M.M., Kirsch, G., Secondary metabolites and biological activity of Pentas species: a minireview. J. Adv. Res. 10:December 2017 (2018), 21–30, 10.1016/j.jare.2017.12.003.
Njoroge, G.N., Bussmann, R.W., Diversity and utilization of antimalarial ethnophytotherapeutic remedies among the Kikuyus (Central Kenya). J. Ethnobiol. Ethnomed. 7 (2006), 1–7, 10.1186/1746-4269-2-8.
Pascaline, J., Charles, M., George, O., Lukhoba, C., Ruth, L., Solomon, D.M., Ethnobotanical survey and propagation of some endangered medicinal plants from south Nandi district of Kenya. J. Anim. Plant Sci. 8:3 (2010), 1016–1043.
El-Hady, S., Bukuru, J., Kesteleyn, B., Van Puyvelde, L., Van Nguyen, T., De Kimpe, N., New pyranonaphthoquinone and pyranonaphthohydroquinone from the roots of Pentas longiflora. J. Nat. Prod., 65(9), 2002.
Bahekar, S., Kale, R., Herbal plants used for the treatment of malaria- a literature review. J. Pharmacogn. Phytochem. 1:6 (2013), 141–146.
Kipkore, W., Wanjohi, B., Rono, H., Kigen, G., A study of the medicinal plants used by the Marakwet Community in Kenya. J. Ethnobiol. Ethnomed., 10(1), 2014, 10.1186/1746-4269-10-24.
Grace, N., Rainer, W., Barbara, G., Eric, N.L., Victoria, N., Utilisation of weed species as sources of traditional medicines in Central Kenya Resumen. Lyonia 7:2 (2004), 71–87.
Esezah, K., Godwin, A., Fredrick, A., Jasper, O.-O., Antifungal medicinal plants used by communities adjacent to Bwindi Impenetrable National Park, South-Western Uganda. Eur. J. Med. Plants 7:4 (2015), 184–192, 10.9734/ejmp/2015/16237.
Ngezahayo, J., Havyarimana, F., Hari, L., Stévigny, C., Duez, P., Medicinal plants used by Burundian traditional healers for the treatment of microbial diseases. J. Ethnopharmacol. 173 (2015), 338–351, 10.1016/j.jep.2015.07.028.
Ngezahayo, J., Havyarimana, F., Hari, L., Stévigny, C., Duez, P., Medicinal plants used by Burundian traditional healers for the treatment of microbial diseases. J. Ethnopharmacol. 173 (2015), 338–351, 10.1016/j.jep.2015.07.028.
Van Puyvelde, L., El Hady, S., De Kimpe, N., Feneau-Dupont, J., Declercq, J.P., Isagarin, a new type of tetracyclic naphthoquinone from the roots of Pentas longiflora. J. Nat. Prod. 61:8 (1998), 1020–1021, 10.1021/np970589o.
Bukuru, J., Isolation and Structural Elucidation of Natural Products from Pentas Bussei K. Krause, Pentas lanceolata (Forsk.) Deflers and Pentas Parvifolia Hiern (Rubiaceae). PhD thesis, 2003.
Van Puyvelde, L., Geysen, D., Ayobangira, F.X., Hakizamungu, E., Nshimyimana, A., Kalisa, A., Screening of medicinal plants of Rwanda for aearicidal activity. J. Ethnopharmacol. 13 (1985), 209–215.
Gao, M., Yang, J., Wang, Z., Yang, B., Kaung, H., Liu, L., Wang, L., Yang, C., Simultaneous determination of purpurin, munjistin and mollugin in rat plasma by ultra high performance liquid chromatography-tandem mass spectrometry: application to a pharmacokinetic study after oral administration of Rubia cordifolia L. extract. Molecules 21:6 (2016), 1–13, 10.3390/molecules21060717.
Nahata, A., Sethiya, N.K., Jain, N., Dixit, V.K., Analysis of scopoletin and mangiferin in botanicals and formulations of Shankhpushpi by HPLC. Herba Pol. 64:4 (2018), 54–62, 10.2478/hepo-2018-0025.
Hubert, P., et al. Harmonization of strategies for the validation of quantitative analytical procedures A SFSTP proposal — part I. J. Pharm. Biomed. Anal. 36 (2004), 579–586, 10.1016/j.jpba.2004.07.027.
Kesteleyn, B., De Kimpe, N., Van Puyvelde, L., Total synthesis of two naphthoquinone antibiotics, psychorubrin and pentalongin, and their C(1)-substituted alkyl and aryl derivatives. J. Organomet. Chem. 64:4 (1999), 1173–1179, 10.1021/jo9811975.
Lemos, A.S.O., et al. Antibacterial and antibiofilm activities of psychorubrin, a pyranonaphthoquinone isolated from Mitracarpus frigidus (Rubiaceae). Front. Microbiol. 9:APR (2018), 1–11, 10.3389/fmicb.2018.00724.
Fabri, R.L., et al. Antitumor, antibiotic and antileishmanial properties of the pyranonaphthoquinone psychorubrin from Mitracarpus frigidus. An. Acad. Bras. Ciências 84:4 (2012), 1081–1089, 10.1590/S0001-37652012005000064.
Harouna, H., et al. Harounoside a pentalongin hydroquinone diglycoside from Mitracarpus scaber. Phytochemistry 39:6 (1995), 1483–1484, 10.1016/0031-9422(95)00088-O.
Audoin, C., et al. MS/MS-guided isolation of clarinoside, a new anti-inflammatory pentalogin derivative. Molecules 23:5 (2018), 1–9, 10.3390/molecules23051237.
WHO, WHO guidelines for selecting marker substances of herbal. WHO Tech. Rep. Ser. No. 1003, 2017, 71–86 [Online]. Available: http://apps.who.int/medicinedocs/en/m/abstract/Js23240en/.
Sánchez-machado, D.I., et al. An HPLC procedure for the quantification of Aloin in latex and gel from Aloe barbadensis leaves. J. Chromatogr. Sci. 55:3 (2017), 251–257, 10.1093/chromsci/bmw179.
Hubert, P., et al. Harmonization of strategies for the validation of quantitative analytical procedures. A SFSTP proposal - part II. J. Pharm. Biomed. Anal. 45:1 (2007), 70–81, 10.1016/j.jpba.2007.06.013.
Rozet, E., et al. Analysis of recent pharmaceutical regulatory documents on analytical method validation. J. Chromatogr. A 1158:1–2 (2007), 111–125, 10.1016/j.chroma.2007.03.111.
Diawara, H.Z., et al. Validation, transfer and measurement uncertainty estimation of an HPLC-UV method for the quantification of artemisinin in hydro alcoholic extracts of Artemisia annua L. J. Pharm. Biomed. Anal. 56:1 (2011), 7–15, 10.1016/j.jpba.2011.04.012.
Marlet, C., Lognay, G., Development and validation by accuracy profile of a method for the analysis of monoterpenes in indoor air by active sampling and thermal desorption-gas chromatography-mass spectrometry. Talanta 82:4 (2010), 1230–1239, 10.1016/j.talanta.2010.06.044.
Lautié, E., Rozet, E., Hubert, P., Quetin Leclercq, J., Quantification of rotenone in seeds of different species of yam bean (Pachyrhizus sp.) by a SPE HPLC-UV method. Food Chem. 131:4 (2012), 1531–1538, 10.1016/j.foodchem.2011.09.125.