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Microbial composition and viability in equine feces after processing for transplantation
Loublier, Clémence; Heinen, Julia; Taminiau, Bernard et al.
20212021 Virtual ACVIM Forum
 

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Keywords :
microbiota; fecal; equine; transplantation; fécale; cheval
Abstract :
[en] Fecal microbiota transplantation has been used empirically for decades in equine medicine but evidence based information on this technique is scarce. This in vitro study aimed to assess the effect of a common fecal pre-transplantation processing method on microbiota composition and viability of the fecal inoculum. A fresh equine fecal sample was divided in three 300g aliquots, to be processed identically as follows: feces (T ) were mixed with 1 L of lukewarm water and chopped using an immersion blender to obtain a mixture (T ), which was left uncovered during 30 minutes (T ) and filtered through a sieve to obtain a fecal infusion (T ). Samples were taken at different steps of the procedure (T ) and immediately stored at 4°C < 6 hours until processing. Bacterial 16S rDNA amplicon profiling associated to propidium-monoazide treatment was performed in each of the 12 samples to select living bacteria. Total flora was measured using quantitative PCR analysis. Bacterial community structure, composition and total flora were statistically compared (significance p < 0.05). No significant differences in ecological indices or abundance of the major bacterial genera were identified for the different steps of fecal processing. Estimated total flora (mean ± SD) in the final fecal infusion was not significantly lower (10.06 ± 0.07 log gene copies/g) than in the original manure (10.29 ± 0.3 log gene copies/g). Results were similar for all three fecal aliquots. These results suggest that the processing method described in this study is replicable and the resultant fecal inoculum contains live bacteria and a microbial population similar to the original fecal sample.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Loublier, Clémence  ;  Université de Liège - ULiège > Département d'Ens. et de Clinique des Equidés > Médecine interne des équidés
Heinen, Julia ;  Université de Liège - ULiège > Département d'Ens. et de Clinique des Equidés > Département d'Ens. et de Clinique des Equidés
Taminiau, Bernard  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Lecoq, Laureline  ;  Université de Liège - ULiège > Département d'Ens. et de Clinique des Equidés > Médecine interne des équidés
Amory, Hélène ;  Université de Liège - ULiège > Département d'Ens. et de Clinique des Equidés > Médecine interne des équidés
Daube, Georges  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires
Cesarini Latorre, Carlota  ;  Université de Liège - ULiège > Département d'Ens. et de Clinique des Equidés > Médecine interne des équidés
Language :
English
Title :
Microbial composition and viability in equine feces after processing for transplantation
Alternative titles :
[fr] Composition et viabilité microbienne dans les feces équines après manipulation pour transplantation
Publication date :
June 2021
Event name :
2021 Virtual ACVIM Forum
Event organizer :
American College of Veterinary Internal Medicine
Event date :
9-12 June 2021
Audience :
International
Available on ORBi :
since 31 May 2021

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