A new isoquinoline and ceramide from the stem barks of Discoglypremna caloneura (Pax) Prain (Euphorbiaceae) with antiproteinase and cytotoxic activities.
Toukam, Paul Djouonzo; Kom, Christelle Wayoue; Tchamgoué, Armelle Deutouet al.
[en] Two new compounds, an isoquinoline (1) and caloneuramide (2), a ceramide were isolated from the stem bark of Discoglypremna caloneura together with seven known compounds namely aurantiamide acetate (3), acetylaleuritolic acid (4), 3α-hydroxylaleuritolic acid 2α-p-hydroxybenzoate (5), mixture of stigmasterol (6) and β-sitosterol (7), mixture of 7-oxo-stigmasterol (8) and 7-oxo-β-sitosterol (9). Their structures were determined based on data from literature and spectroscopic methods. Derivatization reactions on the isoquinoline led to two new compounds, the methylated (10) and acetylated (11) derivatives. Some compounds and extracts were evaluated for their cytotoxic and antiproteinase activity. Antiproteinase effect of compounds 1, 10 and 11 exhibited IC(50) values of 10.77, 1.19 and 3.61 μg/mL respectively; significantly low compared to the standard drug, acetyl salicylic acid (IC(50) = 20.28 μg/mL). Ethyl acetate and methanol extract exhibited moderate cytotoxicity activity on Chang liver cells with CC(50) values of 167.90 ± 2.20 and 106.30 ± 2.03 μg/mL compared to the reference drug cucurmin (CC(50) = 11.05 ± 1.04 μg/mL).
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Toukam, Paul Djouonzo
Kom, Christelle Wayoue
Tchamgoué, Armelle Deutou
Doupno, Estelle Aurelie Kamgang
Lame, Younoussa
Yamthé, Lauve Rachel Tchokouaha
Kopa Kowa, Théodora
Tchinda, Alembert Tiabou
Frederich, Michel ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Mbafor, Joseph Tanyi
De Théodore Atchadé, Alex
Language :
English
Title :
A new isoquinoline and ceramide from the stem barks of Discoglypremna caloneura (Pax) Prain (Euphorbiaceae) with antiproteinase and cytotoxic activities.
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