Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product "Btana".

Abekhti, Abdelkader; Taminiau, Bernard; Nezer, Carine; Hiligsman, Serge; Mabrouk, Kihal; Daube, Georges

doi:10.1007/s10123-021-00166-0

Article (Scientific journals)

Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product "Btana".

Abekhti, Abdelkader; Taminiau, Bernard; Nezer, Carine et al.

2021 • In International microbiology : the official journal of the Spanish Society for Microbiology

Peer reviewed

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https://hdl.handle.net/2268/258274

DOI
10.1007/s10123-021-00166-0

PubMed
33709234

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Keywords :

Btana; Dates; Fermentation; Metabarcoding; Pyrosequencing; Traditional food; Yeasts

Abstract :

[en] The study highlights the fungal diversity of the traditional Algerian date's product "Btana" prepared with direct Btana method (DBM) and undirect Btana method (UBM). Btana fungal populations were analyzed through 28S metabarcoding. Data treatment resulted in 122,997 reads representing three Phyla in which 76% reads (46 OTUs) belong to Ascomycota phylum. Zygosaccharomyces rouxii was the most prevailed species accounting for 35.40% of the total population. Similarity percentage analysis revealed a low level of resemblance in species in each of the two Btana types (DBM: 17.26%, UBM: 16.87). According to HPLC analysis, lactate was detected in nine samples within a range of 0.87-23.06 g/100g. Culture plating and subsequent D1/D2 domain of 28s DNA analysis showed the prevalence of Z. rouxii. Fermentation of non-renewed date medium revealed a high ethanol production (21.31 ± 2.89 g/100g) by Lachancea thermotolerans and 5.87 g/100g of lactates by Kluyveromyces delphensis. Enzymatic assay revealed a high esterase (C4) and naphtol-AS-BI-phosphohydrolase activity by L. thermotolerans, K. delphensis, and Pichia subpelliculosa, while a high level of α-fucosidase was recorded for L. thermotolerans and P. subpelliculosa. The current results demonstrated that the traditional date product Btana is a promising source for yeasts useful in production of value-added products like bioethanol and lactic acid using low-income date cultivars.

Disciplines :

Food science

Author, co-author :

Abekhti, Abdelkader

Taminiau, Bernard ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Nezer, Carine

Hiligsman, Serge

Mabrouk, Kihal

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires (DDA) > Microbiologie des denrées alimentaires

Language :

English

Title :

Metabarcoding analysis and fermentation performance of the dominant fungal microbiota associated with the Algerian traditional date product "Btana".

Publication date :

2021

Journal title :

International microbiology : the official journal of the Spanish Society for Microbiology

ISSN :

1139-6709

eISSN :

1618-1905

Peer reviewed :

Peer reviewed

Available on ORBi :

since 22 March 2021

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