Screening various Yarrowia lipolytica strains for citric acid production

C/N molar ratio; Yarrowia lipolytica; Article; Yarrowia; Batch Cell Culture Techniques; Bioreactors; Citric Acid; Culture Media; Fermentation; Glucose; Nitrogen

Abstract :

[en] Citric acid (CA) productivity by Yarrowia lipolytica dependents on strain type, carbon source, carbon to nitrogen (C/N) molar ratio as well as physicochemical conditions (pH, temperature, oxygen transfer rate, etc.). In the current study, 10 different Y. lipolytica strains were first challenged in shake-flask culture for CA production in a glucose-based media under nitrogen-limited conditions. For the most promising one, strain K57, CA productivity was monitored during culture in batch bioreactor for three initial C/N molar ratio (167, 367, and 567 Cmol/Nmol). The highest CA yield (0.77 g/g glucose), titre (72.3 g/L CA), and productivity (0.04 g/g.h) were found for C/N ratio of 367. However, the highest growth rate was obtained for an initial C/N ratio of 167. From these results, Y. lipolytica strain K57 could be considered to produce CA at higher titre on glucose-based medium in batch bioreactor than others Y. lipolytica strain reported in the literature. © 2019 John Wiley & Sons, Ltd.

Disciplines :

Biotechnology

Author, co-author :

Carsanba, E.; Faculty of Agriculture, Food Engineering Department, Cukurova University, Adana, Turkey, Altinozu Agricultural Sciences Vocational School, Mustafa Kemal University, Antakya, Turkey

Papanikolaou, S.; Department of Food Science& Human Nutrition, Agricultural University of Athens, Athens, Greece

Fickers, Patrick ; Université de Liège - ULiège > Département GxABT > Microbial, food and biobased technologies

Erten, H.; Faculty of Agriculture, Food Engineering Department, Cukurova University, Adana, Turkey

Language :

English

Title :

Screening various Yarrowia lipolytica strains for citric acid production

Publication date :

2019

Journal title :

Yeast

ISSN :

0749-503X

eISSN :

1097-0061

Publisher :

John Wiley and Sons Ltd

Volume :

Issue :

Pages :

319-327

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

FDK‐2014‐3159

Funders :

Cukurova University [TR]

Available on ORBi :

since 18 February 2021

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